ArticleStem cells international2021
Exosomes from LPS-Stimulated hDPSCs Activated the Angiogenic Potential of HUVECs In Vitro.
Article in Stem cells international, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.
What it found
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Who cites it
29 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Role of Lipopolysaccharide, Derived from Various Bacterial Species, in Pulpitis-A Systematic Review.Biomolecules · 2022Pooled it
- Molecular mechanism by which mir-153-3p attenuates pulpitis in human deciduous teeth via PTEN-targeted suppression of inflammation and oxidative stress.Odontology · 2026Article
- Regenerative Dentistry: Applications of Bioactive Materials in Dentin-Pulp Complex.Advances in experimental medicine and biology · 2026Review
- Comparative Analysis of Different Origin-Derived Exosomes for Future Regenerative Endodontic Therapies: A Systematic Review.Clinical, cosmetic and investigational dentistry · 2026Review
- Angiogenic apoptotic vesicle-laden silk fibroin /sodium alginate hydrogel for pulp regeneration.Materials today. Bio · 2025Article
- Regenerative endodontic therapy: From laboratory bench to clinical practice.Journal of advanced research · 2025Review
- Age-Related Oral and Para-Oral Tissue Disorders: The Evolving Therapeutic and Diagnostic Potential of Exosomes.Dentistry journal · 2025Review
- Emerging roles of extracellular vesicles in oral and maxillofacial areas.International journal of oral science · 2025Review
- Angiogenic regulation of dental pulp stem cells.Frontiers in dental medicine · 2025Review
- Tailored Extracellular Vesicles from Dental Stem Cells: Advances in Specific Modifications for Enhanced Therapeutic Applications.International journal of nanomedicine · 2025Review
- Tissue engineering approaches for dental pulp regeneration: The development of novel bioactive materials using pharmacological epigenetic inhibitors.Bioactive materials · 2024Review
- Angiogenic Potential of Various Oral Cavity-Derived Mesenchymal Stem Cells and Cell-Derived Secretome: A Systematic Review and Meta-Analysis.European journal of dentistry · 2024Article
- Exosomes as Promising Therapeutic Tools for Regenerative Endodontic Therapy.Biomolecules · 2024Review
- Advances in Regenerative Dentistry Approaches: An Update.International dental journal · 2024Review
- Emerging roles of exosomes in oral diseases progression.International journal of oral science · 2024Review
- Dental Stem Cell-Derived Exosomes: A Review of Their Isolation, Classification, Functions, and Mechanisms.Stem cells international · 2024Review
- Apoptotic Extracellular Vesicles from Supernumerary Tooth-Derived Pulp Stem Cells Transfer COL1A1 to Promote Angiogenesis via PI3K/Akt/VEGF Pathway.International journal of nanomedicine · 2024Article
- Exosomes derived from odontogenic stem cells: Its role in the dentin-pulp complex.Regenerative therapy · 2023Review
- Crucial Factors Influencing the Involvement of Odontogenic Exosomes in Dental Pulp Regeneration.Stem cell reviews and reports · 2023Review
- Nicotinic Acid-Mediated Modulation of Metastasis-Associated Protein 1 Methylation and Inflammation in Brain Arteriovenous Malformation.Biomolecules · 2023Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundExosomes from human dental pulp stem cells (hDPSCs) were indicated to play a positive role in vascular regeneration processes. But the angiogenic capabilities of exosomes from inflammatory hDPSCs and the underlying mechanism remain unknown. In this study, the inflammatory factor lipopolysaccharide (LPS) was used to stimulate hDPSCs, and exosomes were extracted from these hDPSCs. The proangiogenic potential of exosomes was examined, and the underlying mechanism was studied.
methodExosomes were isolated from hDPSCs with or without LPS stimulation (N-EXO and LPS-EXO) and cocultured with human umbilical vein endothelial cells (HUVECs). The proangiogenic potential of exosomes was evaluated by endothelial cell proliferation, migration, and tube formation abilities in vitro. To investigate the proangiogenic mechanism of LPS-EXO, microRNA sequencing was performed to explore the microRNA profile of N-EXO and LPS-EXO. Gene Ontology (GO) analysis was used to study the functions of the predicted target genes. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis was used to estimate the signaling pathways associated with the inflammation-induced angiogenesis process.
resultCompared to the uptake of N-EXO, uptake of LPS-EXO activated the angiogenic potential of HUVECs by promoting the proliferation, migration, and tube formation abilities in vitro. The mRNA expression levels of vascular endothelial growth factor (VEGF) and kinase-insert domain-containing receptor (KDR) in the LPS-EXO group were significantly higher than those in the N-EXO group. MicroRNA sequencing showed that 10 microRNAs were significantly changed in LPS-EXO. Pathway analysis showed that the genes targeted by differentially expressed microRNAs were involved in multiple angiogenesis-related pathways.
conclusionThis study revealed that exosomes derived from inflammatory hDPSCs possessed better proangiogenic potential in vitro. This is the first time to explore the role of exosomal microRNA from hDPSCs in inflammation-induced angiogenesis. This finding sheds new light on the effect of inflammation-stimulated hDPSCs on tissue regeneration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.