ArticleFrontiers in genetics2021
Three-in-One Simultaneous Extraction of Proteins, Metabolites and Lipids for Multi-Omics.
Article in Frontiers in genetics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Associations Between Follicular Fluid Biomarkers and IVF/ICSI Outcomes in Normo-Ovulatory Women-A Systematic Review.Biomolecules · 2025Pooled it
- π-SeqOmics: A Sequential Workflow for Genomic, Transcriptomic, Proteomic, and Phosphoproteomic Profiling From Biopsy-Scale Samples.Molecular & cellular proteomics : MCP · 2026Article
- Advanced technologies of single-cell metabolomics unveiling cellular metabolic heterogeneity for biological and biomedical research.Journal of food and drug analysis · 2026Review
- Water kefir as a paradigm for multi-omics and genome-scale metabolic modelling in fermented food.NPJ biofilms and microbiomes · 2026Review
- Melatonin promotes stomatal immunity by mediating phytohormone crosstalk inFrontiers in plant science · 2026Article
- The Addition of Transcriptomics to the Bead-Enabled Accelerated Monophasic Multi-Omics Method: A Step toward Universal Sample Preparation.Analytical chemistry · 2024Article
- A diverse proteome is present and enzymatically active in metabolite extracts.Nature communications · 2024Article
- HILIC-IM-MS for Simultaneous Lipid and Metabolite Profiling of Bacteria.ACS measurement science au · 2024Article
- Integrative Metabolomics and Proteomics Allow the Global Intracellular Characterization ofJournal of proteome research · 2024Article
- Optimizing MS-Based Multi-Omics: Comparative Analysis of Protein, Metabolite, and Lipid Extraction Techniques.Metabolites · 2024Article
- The endohyphal microbiome: current progress and challenges for scaling down integrative multi-omic microbiome research.Microbiome · 2023Article
- Metabolomics in paraganglioma: applications and perspectives from genetics to therapy.Endocrine-related cancer · 2023Article
- Creation of a Plant Metabolite Spectral Library for Untargeted and Targeted Metabolomics.International journal of molecular sciences · 2023Article
- Rapid Multi-Omics Sample Preparation for Mass Spectrometry.Analytical chemistry · 2023Article
- Prior metabolite extraction fully preserves RNAseq quality and enables integrative multi-'omics analysis of the liver metabolic response to viral infection.RNA biology · 2023Article
- Untargeted and Targeted LC-MS/MS Based Metabolomics Study on In Vitro Culture ofJournal of fungi (Basel, Switzerland) · 2022Article
- Plant Proteomic Research 4.0: Frontiers in Stress Resilience.International journal of molecular sciences · 2021Article
- Identification of DIR1-Dependant Cellular Responses in Guard Cell Systemic Acquired Resistance.Frontiers in molecular biosciences · 2021Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Elucidation of complex molecular networks requires integrative analysis of molecular features and changes at different levels of information flow and regulation. Accordingly, high throughput functional genomics tools such as transcriptomics, proteomics, metabolomics, and lipidomics have emerged to provide system-wide investigations. Unfortunately, analysis of different types of biomolecules requires specific sample extraction procedures in combination with specific analytical instrumentation. The most efficient extraction protocols often only cover a restricted type of biomolecules due to their different physicochemical properties. Therefore, several sets/aliquots of samples are needed for extracting different molecules. Here we adapted a biphasic fractionation method to extract proteins, metabolites, and lipids from the same sample (3-in-1) for liquid chromatography-tandem mass spectrometry (LC-MS/MS) multi-omics. To demonstrate utility of the improved method, we used bacteria-primed
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