Evidence map›Paper›PMID 33936068›Full record

ArticleFrontiers in immunology2021

Listening to the Whispers in Neuroimmune Crosstalk: A Comprehensive Workflow to Investigate Neurotrophin Receptor p75NTR Under Endogenous, Low Abundance Conditions.

Benjamin W Dorschner, Ralf Wiedemuth, Ann-Christin Funke, Marc Gentzel, Mary-Louise Rogers, Sebastian Brenner, Sebastian Thieme

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in immunology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 95% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 0 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 2 countries.

Benjamin W DorschnerExperimental Hematology, Department of Pediatrics, University Clinic Carl Gustav Carus, Dresden, Germany.
Ralf WiedemuthExperimental Hematology, Department of Pediatrics, University Clinic Carl Gustav Carus, Dresden, Germany.
Ann-Christin FunkeExperimental Hematology, Department of Pediatrics, University Clinic Carl Gustav Carus, Dresden, Germany.
Marc GentzelMolecular Analysis - Mass Spectrometry, Center for Molecular and Cellular Bioengineering (CMCB), Technische Universitaet Dresden, Dresden, Germany.
Mary-Louise RogersCentre for Neuroscience, College of Medicine and Public Health, Flinders University, Adelaide, SA, Australia.
Sebastian BrennerExperimental Hematology, Department of Pediatrics, University Clinic Carl Gustav Carus, Dresden, Germany.
Sebastian ThiemeExperimental Hematology, Department of Pediatrics, University Clinic Carl Gustav Carus, Dresden, Germany.
University Hospital Carl Gustav Carus · DECenter for Systems Biology Dresden · DEFlinders University · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Inflammatory conditions are critically influenced by neuroimmune crosstalk. Cytokines and neurotrophic factors shape the responses of both nervous and immune systems. Although much progress has been made, most findings to date are based on expression of recombinant (tagged) proteins. The examination of receptor interactions by immunoprecipitation (IP) at endogenous levels provides further insight into the more subtle regulations of immune responses. Here, we present a comprehensive workflow and an optimized IP protocol that provide step-by-step instructions to investigate neurotrophin receptor p75NTR at endogenous, low abundance levels: from lysate preparation and confirmation of receptor expression to antibody validation and successful detection of protein-protein interactions. We employ human melanoma cell line A375 to validate specific antibodies and IP conditions, and apply these methods to explore p75NTR interactions in human leukemic plasmacytoid dendritic cell line PMDC05 detecting 14-3-3ϵ:p75NTR interaction in this cell type. With p75NTR as an exemplary protein, our approach provides a strategy to detect specific interaction partners even under endogenous, low abundance expression conditions.

Indexed as

Workflow14-3-3 ProteinsAnimalsAntibodiesBlotting, WesternCell Line, TumorDendritic CellsFlow CytometryFluorescent Antibody TechniqueGene ExpressionHumansHybridomasImmunoprecipitationMass SpectrometryMiceNerve Tissue Proteins14-3-3 ProteinsAntibodiesNerve Tissue ProteinsNGFR protein, humanReceptors, Nerve Growth FactorRNA, MessengerYWHAE protein, humanA375 (human melanoma) cell lineCD271 (p75NTR)endogenous immunoprecipitationmass spectrometryneuroimmune crosstalkp75NTRPMDC05protein-protein interaction

Identifiers

PMID33936068
PMCPMC8085361
OpenAlexW3152940409

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.