ArticleProtoplasma2021
Confocal microscopy reveals alterations of thylakoids in Limnospira fusiformis during prophage induction.
Article in Protoplasma, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Pathogenic effects ofApplied and environmental microbiology · 2026Article
- Viromic and Metagenomic Analyses of Commercial Spirulina Fermentations Reveal Remarkable Microbial Diversity.Viruses · 2024Article
- Automated Segmentation and 3D Reconstruction of Different Membranes from Confocal Z-Stacks.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Volumetric 3D reconstruction of plant leaf cells using SEM, ion milling, TEM, and serial sectioning.Planta · 2022Article
- Ultrastructure of plant cells.Protoplasma · 2021Article
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Authors and funding
4 authors.
Funding
Abstract
The alkaliphilic cyanobacterium Limnospira fusiformis is an integral part in food webs of tropical soda lakes. Recently, sudden breakdowns of Limnospira sp. blooms in their natural environment have been linked to cyanophage infections. We studied ultrastructural details and prophage components in the laboratory by means of confocal laser scanning microscopy (CLSM) and transmission electron microscopy (TEM). For a comparison at the subcellular level, we included transmission electron microscopy (TEM) material of infected cells collected during a field survey. Compared to TEM, CLSM has the advantage to rapidly providing results for whole, intact cells. Moreover, many cells can be studied at once. We chemically induced lysogenic cyanophages by means of mitomycin C (MMC) treatments and studied the ultrastructural alterations of host cells. In parallel, the number of cyanophages was obtained by flow cytometry. After treatment of the culture with MMC, flow cytometry showed a strong increase in viral counts, i.e., prophage induction. CLSM reflected the re-organization of L. fusiformis with remarkable alterations of thylakoid arrangements after prophage induction. Our study provides a first step towards 3D visualization of ultrastructure of cyanobacteria and showed the high potential of CLSM to investigate viral-mediated modifications in these groups.
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