ArticleParasites & vectors2021
Assessing the suitability of mitochondrial and nuclear DNA genetic markers for molecular systematics and species identification of helminths.
Article in Parasites & vectors, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers, 1 of them a synthesis that pooled it.
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Who cites it
45 citing papers in PubMed, 1 synthesis or guideline pooled it, 83 citations in OpenAlex.
- Transforming gastrointestinal helminth parasite identification in vertebrate hosts with metabarcoding: a systematic review.Parasites & vectors · 2024Pooled it
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- Molecular Epidemiology of Helminths at the Wildlife-Livestock Interface in Kazakhstan: Evidence from Sheep and Saiga.Pathogens (Basel, Switzerland) · 2026Article
- Polymorphic marker regions support divergence of Mansonella sp. "DEUX" and M. perstans.Parasites & vectors · 2026Article
- Phylogenetic and haplotype network analysis of Ixodes crenulatus, Ixodes canisuga, Ixodes acuminatus and Ixodes redikorzevi (subgenus Pholeoixodes) from their geographical range, Europe and China.Experimental & applied acarology · 2026Article
- On species boundaries in chigger mites: an integrative approach to Hirsutiella spp. (Trombiculidae) in Poland.Experimental & applied acarology · 2026Article
- Mitochondrial DNA phylogeography of a species-specific sucking louse, Johnsonpthirus heliosciuri, act as a proxy to provide insights into the population connectivity of its host, smith's bush squirrels, Paraxerus cepapi.Parasitology research · 2026Article
- New insights intoFrontiers in veterinary science · 2026Article
- Next-generation molecular tools in veterinary parasitology: advances, challenges, and perspectives in the diagnosis of emerging parasites.Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria · 2026Review
- Genetic diversity and geographic distribution of Fasciola species from cattle in eight provinces of South Africa.Scientific reports · 2025Article
- Hidden in morphology, revealed by molecular genetics: synonymization ofParasitology · 2025Article
- Natural infection of filarioid nematodes in mosquitoes: An approach to neglected disease xenosurveillance and prevention in intercontinental human transit areas in Darién.PLoS neglected tropical diseases · 2025Article
- A NovelBiomolecules · 2025Article
- Morphological and molecular assessment of muscle metacercariae infecting tench Tinca tinca from fish farms and wild populations in Germany.Scientific reports · 2025Article
- First complete mitochondrial genome of Armillifer moniliformis (Pentastomida: Porocephalida) isolated from a human case in Northern Thailand: comparative and phylogenetic analyses.Parasitology research · 2025Article
- Validating a web application's use of genetic distance to determine helminth species boundaries and aid in identification.BMC bioinformatics · 2025Article
- Integrative taxonomy in helminth analysis: protocols and limitations in the twenty-first century.Parasites & vectors · 2025Review
- High-Throughput Sequencing Enables Rapid Analyses of Nematode Mitochondrial Genomes from an Environmental Sample.Pathogens (Basel, Switzerland) · 2025Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundGenetic markers are employed widely in molecular studies, and their utility depends on the degree of sequence variation, which dictates the type of application for which they are suited. Consequently, the suitability of a genetic marker for any specific application is complicated by its properties and usage across studies. To provide a yardstick for future users, in this study we assess the suitability of genetic markers for molecular systematics and species identification in helminths and provide an estimate of the cut-off genetic distances per taxonomic level.
methodsWe assessed four classes of genetic markers, namely nuclear ribosomal internal transcribed spacers, nuclear rRNA, mitochondrial rRNA and mitochondrial protein-coding genes, based on certain properties that are important for species identification and molecular systematics. For molecular identification, these properties are inter-species sequence variation; length of reference sequences; easy alignment of sequences; and easy to design universal primers. For molecular systematics, the properties are: average genetic distance from order/suborder to species level; the number of monophyletic clades at the order/suborder level; length of reference sequences; easy alignment of sequences; easy to design universal primers; and absence of nucleotide substitution saturation. Estimation of the cut-off genetic distances was performed using the 'K-means' clustering algorithm.
resultsThe nuclear rRNA genes exhibited the lowest sequence variation, whereas the mitochondrial genes exhibited relatively higher variation across the three groups of helminths. Also, the nuclear and mitochondrial rRNA genes were the best possible genetic markers for helminth molecular systematics, whereas the mitochondrial protein-coding and rRNA genes were suitable for molecular identification. We also revealed that a general gauge of genetic distances might not be adequate, using evidence from the wide range of genetic distances among nematodes.
conclusionThis study assessed the suitability of DNA genetic markers for application in molecular systematics and molecular identification of helminths. We provide a novel way of analyzing genetic distances to generate suitable cut-off values for each taxonomic level using the 'K-means' clustering algorithm. The estimated cut-off genetic distance values, together with the summary of the utility and limitations of each class of genetic markers, are useful information that can benefit researchers conducting molecular studies on helminths.
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