ArticleMicrobial cell factories2021
Surface display as a functional screening platform for detecting enzymes active on PET.
Article in Microbial cell factories, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 35 citations in OpenAlex.
- Megascale recombination generates millions of catalytically competent PETases.bioRxiv : the preprint server for biology · 2026Article
- Plastic additives enrich diverse bacterial communities which show the hallmarks of plastic degradation.Sustainable microbiology · 2026Article
- Repurposing the bacterial surface display technology for drug delivery.Advanced drug delivery reviews · 2025Review
- Engineered Yeasts Displaying PETase and MHETase as Whole-Cell Biocatalysts for the Degradation of Polyethylene Terephthalate (PET).ACS synthetic biology · 2025Article
- Standardization guidelines and future trends for PET hydrolase research.Nature communications · 2025Review
- Bacillus subtilis surface display technology: applications in bioprocessing and sustainable manufacturing.Biotechnology for biofuels and bioproducts · 2025Review
- Efficient biodegradation and upcycling of polyethylene terephthalate mediated by cell-factories.Frontiers in microbiology · 2025Review
- Biodegradation of combined pollutants of polyethylene terephthalate and phthalate esters by esterase-integratedSynthetic and systems biotechnology · 2025Article
- Harnessing Nature-Inspired Catechol Amino Acid to Engineer Sticky Proteins and Bacteria.Small methods · 2024Article
- A versatile microbial platform as a tunable whole-cell chemical sensor.Nature communications · 2024Article
- Article
- Harnessing extremophilic carboxylesterases for applications in polyester depolymerisation and plastic waste recycling.Essays in biochemistry · 2023Article
- Emerging enzyme surface display systems for waste resource recovery.Environmental microbiology · 2023Review
- Towards synthetic PETtrophy: Engineering Pseudomonas putida for concurrent polyethylene terephthalate (PET) monomer metabolism and PET hydrolase expression.Microbial cell factories · 2022Article
- Biochemical characterization of a polyethylene terephthalate hydrolase and design of high-throughput screening for its directed evolution.Engineering microbiology · 2022Article
- Mechanism-Based Design of Efficient PET Hydrolases.ACS catalysis · 2022Review
- A Modular System for the Rapid Comparison of Different Membrane Anchors for Surface Display on Escherichia coli.Chembiochem : a European journal of chemical biology · 2022Article
- New approaches for the characterization of plastic-associated microbial communities and the discovery of plastic-degrading microorganisms and enzymes.Computational and structural biotechnology journal · 2021Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Poly(ethylene terephthalate) (PET) is the world's most abundant polyester plastic, and its ongoing accumulation in nature is causing a global environmental problem. Currently, the main recycling processes utilize thermomechanical or chemical means, resulting in the deterioration of the mechanical properties of PET. Consequently, polluting de novo synthesis remains preferred, creating the need for more efficient and bio-sustainable ways to hydrolyze the polymer. Recently, a PETase enzyme from the bacterium Ideonella sakaiensis was shown to facilitate PET biodegradation, albeit at slow rate. Engineering of more efficient PETases is required for industrial relevance, but progress is currently hampered by the dependency on intracellular expression in Escherichia coli. To create a more efficient screening platform in E. coli, we explore different surface display anchors for fast and easy assaying of PETase activity. We show that PETases can be functionally displayed on the bacterial cell surface, enabling screening of enzyme activity on PET microparticles - both while anchored to the cell and following solubilization of the enzymes.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.