Evidence map›Paper›PMID 33932437›Full record

ArticleBiophysical journal2021

Determination of bacterial surface charge density via saturation of adsorbed ions.

Michael J Wilhelm, Mohammad Sharifian Gh, Tong Wu, Yujie Li, Chia-Mei Chang, Jianqiang Ma, Hai-Lung Dai

Abstract read
In one paragraph

Article in Biophysical journal, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.

0numbers the graph read from it
0cells of the map it votes in
34citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

34 citing papers in PubMed.

  1. Article
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  3. ZrOJournal of functional biomaterials · 2026
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  7. Review
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  13. Article
  14. Bacteria-Mediated Intracellular Radical Polymerizations.Journal of the American Chemical Society · 2025
    Article
  15. Review
  16. Quantifying bacterial efflux within subcellular domains ofApplied and environmental microbiology · 2024
    Article
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Michael J WilhelmDepartment of Chemistry, Temple University, Philadelphia, Pennsylvania. Electronic address: michael.wilhelm@temple.edu.
Mohammad Sharifian GhDepartment of Chemistry, Temple University, Philadelphia, Pennsylvania.
Tong WuDepartment of Chemistry, Temple University, Philadelphia, Pennsylvania.
Yujie LiDepartment of Chemistry, Temple University, Philadelphia, Pennsylvania.
Chia-Mei ChangDepartment of Chemistry, Temple University, Philadelphia, Pennsylvania.
Jianqiang MaDepartment of Chemistry, Temple University, Philadelphia, Pennsylvania.
Hai-Lung DaiDepartment of Chemistry, Temple University, Philadelphia, Pennsylvania.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Bacterial surface charge is a critical characteristic of the cell's interfacial physiology that influences how the cell interacts with the local environment. A direct, sensitive, and accurate experimental technique capable of quantifying bacterial surface charge is needed to better understand molecular adaptations in interfacial physiology in response to environmental changes. We introduce here the method of second-harmonic light scattering (SHS), which is capable of detecting the number of molecular ions adsorbed as counter charges on the exterior bacterial surface, thereby providing a measure of the surface charge. In this first demonstration, we detect the small molecular cation, malachite green, electrostatically adsorbed on the surface of representative strains of Gram-positive and Gram-negative bacteria. Surprisingly, the SHS-deduced molecular transport rates through the different cellular ultrastructures are revealed to be nearly identical. However, the adsorption saturation densities on the exterior surfaces of the two bacteria were shown to be characteristically distinct. The negative charge density of the lipopolysaccharide coated outer surface of Gram-negative Escherichia coli (6.6 ± 1.3 nm

Indexed as

Anti-Bacterial AgentsGram-Negative BacteriaAdsorptionGram-Positive BacteriaIonsSurface PropertiesAnti-Bacterial AgentsIons

Identifiers

PMID33932437
PMCPMC8390864

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.