ArticleNature communications2021
Single nucleus RNA-sequencing defines unexpected diversity of cholinergic neuron types in the adult mouse spinal cord.
Article in Nature communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 99 papers.
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Who cites it
99 citing papers in PubMed, 147 citations in OpenAlex.
- A who's who of cell types in skeletal muscle.Communications biology · 2026Review
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- Transcriptomic analysis of spinal V1 interneurons informs their multifunctional role in motor output.Nature communications · 2026Article
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- Distinct transcriptional programs define cranial motor neuron subtypes during vertebrate development.bioRxiv : the preprint server for biology · 2026Article
- Single-nucleus RNA sequencing reveals cell type-specific responses to heat stress in bovine mammary gland.Journal of animal science and biotechnology · 2026Article
- Deep Cellular and Spatial Profiling of the Mouse Spinal Cord Reveals Sex-Specific Neuron Types and the Ascending Projection Neuron Repertoire.bioRxiv : the preprint server for biology · 2026Article
- Ion channel and receptor-mediated regulation of axonal conduction reliability in sympathetic preganglionic neurons.bioRxiv : the preprint server for biology · 2026Article
- Probabilistic spike propagation shapes sympathetic output in mouse preganglionic neurons.bioRxiv : the preprint server for biology · 2026Article
- Temperature and frequency dependence of conduction along sympathetic preganglionic axons.bioRxiv : the preprint server for biology · 2026Article
- Redefining the central pattern generator for vertebrate locomotion.Nature reviews. Neuroscience · 2026Review
- Transcriptional and cellular maturation of the chick spinal cord in the context of distinct neuromuscular circuits.iScience · 2026Article
- Little Known Facts, Controversies and Misconceptions About Cranial Motor Nuclei (and a New Classification for All Motoneurons, Cranial, and Spinal).The Journal of comparative neurology · 2026Review
- Whole-Brain Neural Connectivity to Cholinergic Neurons in the Lower Thoracic Intermediolateral Column.CNS neuroscience & therapeutics · 2026Article
- Decoding neurodegeneration one cell at a time.The Journal of clinical investigation · 2026Review
- A consensus spinal cord cell type atlas across mouse, macaque, and human.bioRxiv : the preprint server for biology · 2026Article
- Mesoscopic analysis of GABAergic marker expression in acetylcholine neurons in the whole mouse brain.iScience · 2026Article
- Axonal Eif5a hypusination controls local translation and mitigates defects in FUS-ALS.Nature neuroscience · 2026Article
- Impaired dynein function preserves spinal interneuron survival and positioning in an ALS-like mouse model.PloS one · 2026Article
- Fentanyl blockade of KThe Journal of physiology · 2026Article
39 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
In vertebrates, motor control relies on cholinergic neurons in the spinal cord that have been extensively studied over the past hundred years, yet the full heterogeneity of these neurons and their different functional roles in the adult remain to be defined. Here, we develop a targeted single nuclear RNA sequencing approach and use it to identify an array of cholinergic interneurons, visceral and skeletal motor neurons. Our data expose markers for distinguishing these classes of cholinergic neurons and their rich diversity. Specifically, visceral motor neurons, which provide autonomic control, can be divided into more than a dozen transcriptomic classes with anatomically restricted localization along the spinal cord. The complexity of the skeletal motor neurons is also reflected in our analysis with alpha, gamma, and a third subtype, possibly corresponding to the elusive beta motor neurons, clearly distinguished. In combination, our data provide a comprehensive transcriptomic description of this important population of neurons that control many aspects of physiology and movement and encompass the cellular substrates for debilitating degenerative disorders.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.