ArticleHorticulture research2021
Whole-genome sequencing reveals rare off-target mutations in CRISPR/Cas9-edited grapevine.
Article in Horticulture research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
27 citing papers in PubMed, 61 citations in OpenAlex.
- Nanotechnology-Enabled CRISPR Delivery: Emerging Opportunities in Agriculture and Forest Biotechnology.Plants (Basel, Switzerland) · 2026Review
- High-throughput Raman-activated cell sorting of microalgal genome-wide edited library revealed a regulatory pathway for carotenoid synthesis.Nature communications · 2026Article
- Root-Specific Signal Modules Mediating Abiotic Stress Tolerance in Fruit Crops.Plants (Basel, Switzerland) · 2026Review
- Trans-generational maintenance of mitochondrial DNA integrity in oocytes during early folliculogenesis.PLoS genetics · 2025Article
- Design principle of successful genome editing applications using CRISPR-based toolkits.Journal of applied genetics · 2025Review
- In Vitro Regeneration of Southern Italian Grapevine Cultivars from Embryogenic Calluses and Protoplasts.Plants (Basel, Switzerland) · 2025Article
- Review
- CRISPR-Cas Gene Editing Technology in Potato.International journal of molecular sciences · 2025Review
- Advances in the molecular mechanism of grapevine resistance to fungal diseases.Molecular horticulture · 2025Review
- Single-stranded DNA (ssDNA) donor repair templates and CRISPR/Cas9 enable a high-frequency of targeted insertions in potato.Frontiers in genome editing · 2025Article
- Development and Applications of Somatic Embryogenesis in Grapevine (Plants (Basel, Switzerland) · 2024Review
- Impact of predictive selection of LbCas12a CRISPR RNAs upon on- and off-target editing rates in soybean.Plant direct · 2024Article
- CRISPR/Cas9 revolutionizesHorticulture research · 2024Article
- Telomere-to-telomere and gap-free genome assembly of a susceptible grapevine species (Thompson Seedless) to facilitate grape functional genomics.Horticulture research · 2024Article
- Rare but diverse off-target and somatic mutations found in field and greenhouse grown trees expressing CRISPR/Cas9.Frontiers in bioengineering and biotechnology · 2024Article
- Genome-wide evaluation of gene editing outcomes using CRISPR/Cas9 in seed propagatedFrontiers in plant science · 2024Article
- Review
- Genome editing in plants using the compact editor CasΦ.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- CRISPR/Cas9 Technology for Potato Functional Genomics and Breeding.Methods in molecular biology (Clifton, N.J.) · 2023Article
- VCFshiny: an R/Shiny application for interactively analyzing and visualizing genetic variants.Bioinformatics advances · 2023Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
Abstract
The CRISPR (clustered regularly interspaced short palindromic repeats)-associated protein 9 (Cas9) system is a powerful tool for targeted genome editing, with applications that include plant biotechnology and functional genomics research. However, the specificity of Cas9 targeting is poorly investigated in many plant species, including fruit trees. To assess the off-target mutation rate in grapevine (Vitis vinifera), we performed whole-genome sequencing (WGS) of seven Cas9-edited grapevine plants in which one of two genes was targeted by CRISPR/Cas9 and three wild-type (WT) plants. In total, we identified between 202,008 and 272,397 single nucleotide polymorphisms (SNPs) and between 26,391 and 55,414 insertions/deletions (indels) in the seven Cas9-edited grapevine plants compared with the three WT plants. Subsequently, 3272 potential off-target sites were selected for further analysis. Only one off-target indel mutation was identified from the WGS data and validated by Sanger sequencing. In addition, we found 243 newly generated off-target sites caused by genetic variants between the Thompson Seedless cultivar and the grape reference genome (PN40024) but no true off-target mutations. In conclusion, we observed high specificity of CRISPR/Cas9 for genome editing of grapevine.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.