ArticleApplied and environmental microbiology2021
A New Pathway for Forming Acetate and Synthesizing ATP during Fermentation in Bacteria.
Article in Applied and environmental microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 45 citations in OpenAlex.
- The anaerobic fungusmBio · 2026Article
- Dietary Fiber-Associated Differences in Gut Microbial Community Composition and Predicted Short-Chain Fatty Acid-Related Functional Potential: An In Silico Re-Analysis of 16S rRNA Data.International journal of molecular sciences · 2026Article
- Succinate and lactate produced as conserved biomarkers through chronic and transient substrate-level phosphorylation: from microorganisms to cancer.Journal of bioenergetics and biomembranes · 2026Review
- Acetate-Linked Energy Metabolism as a Determinant of Early Haemophilus influenzae Infection Fitness.MicrobiologyOpen · 2026Article
- Short-chain fatty acids-a key link between the gut microbiome and T-lymphocytes in neonates?Pediatric research · 2026Review
- Red seaweed supplementation suppresses methanogenesis in the rumen, revealing potentially advantageous traits among hydrogenotrophic bacteria.Microbiome · 2025Article
- Insights into Microbial and Metabolite Profiles in Traditional Northern Thai Fermented Soybean (Tuanao) Fermentation Through Metagenomics and Metabolomics.Foods (Basel, Switzerland) · 2025Article
- Metabolic ecology of microbiomes: Nutrient competition, host benefits, and community engineering.Cell host & microbe · 2025Review
- Isolation ofJDS communications · 2025Article
- The vast landscape of carbohydrate fermentation in prokaryotes.FEMS microbiology reviews · 2024Review
- New biochemical pathways for forming short-chain fatty acids during fermentation in rumen bacteria.JDS communications · 2024Article
- Systemic metabolic depletion of gut microbiome undermines responsiveness to melanoma immunotherapy.Life science alliance · 2024Article
- Bacterial acetate metabolism and its influence on human epithelia.Emerging topics in life sciences · 2024Review
- Swinging between the beneficial and harmful microbial community in biofloc technology: A paradox.Heliyon · 2024Review
- Gene Expression of Ethanol and Acetate Metabolic Pathways in theMicroorganisms · 2024Article
- Tracing active members in microbial communities by BONCAT and click chemistry-based enrichment of newly synthesized proteins.ISME communications · 2024Article
- Elevated acetate kinase (ackA) gene expression, activity, and biofilm formation observed in methicillin-resistant strains of Staphylococcus aureus (MRSA).Journal, genetic engineering & biotechnology · 2023Article
- The oxidoreductase activity of Rnf balances redox cofactors during fermentation of glucose to propionate in Prevotella.Scientific reports · 2023Article
- The phenotype and genotype of fermentative prokaryotes.Science advances · 2023Article
- Genome-scale metabolic modeling andFrontiers in cellular and infection microbiology · 2023Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many bacteria and other organisms carry out fermentations forming acetate. These fermentations have broad importance for foods, agriculture, and industry. They also are important for bacteria themselves because they often generate ATP. Here, we found a biochemical pathway for forming acetate and synthesizing ATP that was unknown in fermentative bacteria. We found that the bacterium Cutibacterium granulosum formed acetate during fermentation of glucose. It did not use phosphotransacetylase or acetate kinase, enzymes found in nearly all acetate-forming bacteria. Instead, it used a pathway involving two different enzymes. The first enzyme, succinyl coenzyme A (succinyl-CoA):acetate CoA-transferase (SCACT), forms acetate from acetyl-CoA. The second enzyme, succinyl-CoA synthetase (SCS), synthesizes ATP. We identified the genes encoding these enzymes, and they were homologs of SCACT and SCS genes found in other bacteria. The pathway resembles one described in eukaryotes, but it uses bacterial, not eukaryotic, gene homologs. To find other instances of the pathway, we analyzed sequences of all biochemically characterized homologs of SCACT and SCS (103 enzymes from 64 publications). Homologs with similar enzymatic activity had similar sequences, enabling a large-scale search for them in genomes. We searched nearly 600 genomes of bacteria known to form acetate, and we found that 6% encoded homologs with SCACT and SCS activity. This included >30 species belonging to 5 different phyla, showing that a diverse range of bacteria encode the SCACT/SCS pathway. This work suggests the SCACT/SCS pathway is important for acetate formation in many branches of the tree of life.
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