Evidence map›Paper›PMID 33931072›Full record

ArticleBMC veterinary research2021

qPCR assays with dual-labeled probes for genotyping honey bee variants associated with varroa resistance.

David Claeys Boúúaert, Mario Van Poucke, Lina De Smet, Wim Verbeke, Dirk C de Graaf, Luc Peelman

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC veterinary research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.4field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

David Claeys Boúúaert *Laboratory of Molecular Entomology and Bee Pathology, Ghent University, Krijgslaan 281, B-9000, Ghent, Belgium. david.claeysbouuaert@ugent.be.ORCID http://orcid.org/0000-0001-8712-5882
Mario Van Poucke *Animal Genetics Laboratory, Ghent University, Heidestraat 19, B-9820, Merelbeke, Belgium.
Lina De SmetLaboratory of Molecular Entomology and Bee Pathology, Ghent University, Krijgslaan 281, B-9000, Ghent, Belgium.
Wim VerbekeDepartment of Agricultural Economics, Ghent University, Coupure links 653, B-9000, Ghent, Belgium.
Dirk C de GraafLaboratory of Molecular Entomology and Bee Pathology, Ghent University, Krijgslaan 281, B-9000, Ghent, Belgium.
Luc PeelmanAnimal Genetics Laboratory, Ghent University, Heidestraat 19, B-9820, Merelbeke, Belgium.
Ghent University · BE

Funding

FOD Volksgezondheid, Veiligheid van de Voedselketen en Leefmilieu RT19/6336Horizon 2020 No 817622
6 · The paper itself

Abstract

backgroundThe varroa mite is one of the main causes of honey bee mortality. An important mechanism by which honey bees increase their resistance against this mite is the expression of suppressed mite reproduction. This trait describes the physiological inability of mites to produce viable offspring and was found associated with eight genomic variants in previous research.

resultsThis paper presents the development and validation of high-throughput qPCR assays with dual-labeled probes for discriminating these eight single-nucleotide variants. Amplicon sequences used for assay validation revealed additional variants in the primer/probe binding sites in four out of the eight assays. As for two of these the additional variants interfered with the genotyping outcome supplementary primers and/or probes were developed. Inclusion of these primers and probes in the assay mixes allowed for the correct genotyping of all eight variants of interest within our bee population.

conclusionThese outcomes underline the importance of checking for interfering variants in designing qPCR assays. Ultimately, the availability of this assay allows genotyping for the suppressed mite reproduction trait and paves the way for marker assisted selection in breeding programs.

Indexed as

AnimalsBeesGenotypeHigh-Throughput Nucleotide SequencingHost-Parasite InteractionsReal-Time Polymerase Chain ReactionReproductionVarroidaeHigh-throughput DNA testHoney beeResilienceSuppressed mite reproductionVarroa destructorVarroa resistance

Identifiers

PMID33931072
PMCPMC8086294
OpenAlexW3127670466

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.