Evidence map›Paper›PMID 33930819›Full record

ArticleVirology2021

Expression of SARS coronavirus 1 spike protein from a herpesviral vector induces innate immune signaling and neutralizing antibody responses.

Evelyn A Kurt-Jones, Timothy E Dudek, Daisuke Watanabe, Leisa Mandell, Jenny Che, Shenghua Zhou, LuCheng Cao, Thomas Greenough, Gregory J Babcock, Fernando Diaz and 4 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Virology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.6field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. The Quest for Immunity: Exploring Human Herpesviruses as Vaccine Vectors.International journal of molecular sciences · 2023
    Review
  3. Review
  4. A virus-specific monocyte inflammatory phenotype is induced by SARS-CoV-2 at the immune-epithelial interface.Proceedings of the National Academy of Sciences of the United States of America · 2022
    Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 2 institutions in 1 country.

Evelyn A Kurt-JonesDepartment of Medicine, University of Massachusetts Medical School, Worcester, MA, USA.
Timothy E DudekDepartment of Microbiology, Blavatnik Institute, Harvard Medical School, Boston, MA, USA.
Daisuke WatanabeDepartment of Microbiology, Blavatnik Institute, Harvard Medical School, Boston, MA, USA.
Leisa MandellDepartment of Medicine, University of Massachusetts Medical School, Worcester, MA, USA.
Jenny CheDepartment of Medicine, University of Massachusetts Medical School, Worcester, MA, USA.
Shenghua ZhouDepartment of Medicine, University of Massachusetts Medical School, Worcester, MA, USA.
LuCheng CaoDepartment of Medicine, University of Massachusetts Medical School, Worcester, MA, USA.
Thomas GreenoughDepartment of Medicine, University of Massachusetts Medical School, Worcester, MA, USA.
Gregory J BabcockMassBiologics, University of Massachusetts Medical School, Boston, MA, USA.
Fernando DiazDepartment of Microbiology, Blavatnik Institute, Harvard Medical School, Boston, MA, USA.
Hyung Suk OhDepartment of Microbiology, Blavatnik Institute, Harvard Medical School, Boston, MA, USA.
Changhong ZhouDepartment of Microbiology, Blavatnik Institute, Harvard Medical School, Boston, MA, USA.
Robert W FinbergDepartment of Medicine, University of Massachusetts Medical School, Worcester, MA, USA.
David M KnipeDepartment of Microbiology, Blavatnik Institute, Harvard Medical School, Boston, MA, USA. Electronic address: david_knipe@hms.harvard.edu.
University of Massachusetts Chan Medical School · USHarvard University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

SARS coronavirus 1 (SARS-CoV-1) causes a respiratory infection that can lead to acute respiratory distress characterized by inflammation and high levels of cytokines in the lung tissue. In this study we constructed a herpes simplex virus 1 replication-defective mutant vector expressing SARS-CoV-1 spike protein as a potential vaccine vector and to probe the effects of spike protein on host cells. The spike protein expressed from this vector is functional in that it localizes to the surface of infected cells and induces fusion of ACE2-expressing cells. In immunized mice, the recombinant vector induced antibodies that bind to spike protein in an ELISA assay and that show neutralizing activity. The spike protein expressed from this vector can induce the expression of cytokines in an ACE2-independent, MyD88-dependent process. These results argue that the SARS-CoV-1 spike protein intrinsically activates signaling pathways that induce cytokines and contribute directly to the inflammatory process of SARS.

Indexed as

Immunity, InnateAnimalsAntibodies, NeutralizingAntibodies, ViralCell FusionCell LineCytokinesGenetic VectorsHerpesvirus 1, HumanHumansMiceSevere acute respiratory syndrome-related coronavirusSignal TransductionSpike Glycoprotein, CoronavirusVaccines, Virus-Like ParticleViral VaccinesAntibodies, NeutralizingAntibodies, ViralCytokinesSpike Glycoprotein, Coronavirusspike glycoprotein, SARS-CoVVaccines, Virus-Like ParticleViral VaccinesCytokinesHerpes simplex virusSARS coronavirus

Identifiers

PMID33930819
PMCPMC8058630
OpenAlexW3155069453

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.