ReviewPathology, research and practice2021
Recent advances and challenges of RT-PCR tests for the diagnosis of COVID-19.
Review in Pathology, research and practice, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 101 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
101 citing papers in PubMed.
- Multiplexed detection of febrile infections using CARMEN.Nature communications · 2025Trial
- Review
- Association between clinical symptoms and time to pharyngeal PCR negativity among mild omicron COVID-19 patients: a retrospective study.BMC infectious diseases · 2026Article
- Extraoral Detection of Biomarkers and Pathogens in Saliva: Comprehensive, Panoramic Review.Biosensors · 2026Review
- An Oscillating-Flow Microfluidic PCR Method for Rapid and Flexible Detection of Periodontal Pathogens.Sensors (Basel, Switzerland) · 2026Article
- Learning from pandemics: Feature selection and clustering for robust digital disease nowcasting.PloS one · 2026Article
- Preemptive rapid antigen test as an emergency measure during coronavirus disease 2019 outbreaks.Frontiers in public health · 2026Article
- Metagenomic profiling of the gut microbiome to predict orthopedic healing responses in postmenopausal women.Frontiers in cellular and infection microbiology · 2026Article
- Research Trends and Development Dynamics of qPCR-based Biomarkers: A Comprehensive Bibliometric Analysis.Molecular biotechnology · 2026Review
- Light-induced field effect transistor-based biosensor consisted of aptamer and ReSMaterials today. Bio · 2025Article
- Tracking the pandemic through molecular and sequencing tools: a story of SARS- CoV-2 over five years, lessons learned, and further directions.BMC infectious diseases · 2025Article
- Modeling the effectiveness of RT-PCR, RT-LAMP, and antigen testing strategies for COVID-19 control.BMC infectious diseases · 2025Article
- Development of a Microfluidic Point-of-Care Platform for HPV Detection Based on Helicase-Dependent Amplification.Tropical medicine and infectious disease · 2025Article
- Review
- Fast, Simple Electrochromic Biosensors for Point-of-Use Infectious Disease Diagnosis.Analytical chemistry · 2025Article
- Democratization of Point-of-Care Viral Biosensors: Bridging the Gap from Academia to the Clinic.Biosensors · 2025Review
- Review
- Immobilizing and Patterning DNA on Simplified Protein-Free DNA-Based Lateral Flow Assays.ACS omega · 2025Article
- Molecular-based laboratory testing confer accuracy over microscopical testing for tick identification.International journal for parasitology. Parasites and wildlife · 2025Article
- Implementing SARS-CoV-2 Testing during a Large-Scale Sporting Event in Africa: Lessons Learned from the Africa Football Cup of Nations Tournament in Cameroon.The American journal of tropical medicine and hygiene · 2025Article
41 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Since the outbreak of the novel severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the control of virus spread has remained challenging given the pitfalls of the current diagnostic tests. Nevertheless, RNA amplification techniques have been the gold standard among other diagnostic methods for monitoring clinical samples for the presence of the virus. In the current paper, we review the shortcomings and strengths of RT-PCR (real-time polymerase chain reaction) techniques for diagnosis of coronavirus disease (COVID)-19. We address the repercussions of false-negative and false-positive rates encountered in the test, summarize approaches to improve the overall sensitivity of this method. We discuss the barriers to the widespread use of the RT-PCR test, and some technical advances, such as RT-LAMP (reverse-transcriptase-loop mediated isothermal amplification). We also address how other molecular techniques, such as immunodiagnostic tests can be used to avoid incorrect interpretation of RT-PCR tests.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.