ArticleJournal of endocrinological investigation2021
Molecular diagnosis of SARS-CoV-2 in seminal fluid.
Article in Journal of endocrinological investigation, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 3 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
21 citing papers in PubMed, 3 syntheses or guidelines pooled it, 28 citations in OpenAlex.
- Pooled it
- The effect of COVID-19 vaccines on sperm parameters: a systematic review and meta-analysis.Asian journal of andrology · 2023Pooled it
- Andrological effects of SARS-Cov-2 infection: a systematic review and meta-analysis.Journal of endocrinological investigation · 2022Pooled it
- Article
- Repercussion of SARS-CoV-2 on the Sexual Function in Males: An Updated Review.Infectious disorders drug targets · 2025Review
- SARS-CoV-2 in semen: a multicenter prospective study and literature review.Basic and clinical andrology · 2024Article
- Molecular study of the presence and transcriptional activity of HPV in semen.Journal of endocrinological investigation · 2024Article
- Persistent oligonecrozoospermia after asymptomatic SARS-CoV-2 infection. A case report and literature review.Heliyon · 2023Article
- Viruses, bacteria and parasites: infection of the male genital tract and fertility.Basic and clinical andrology · 2023Review
- Male Reproductive Tract Involvement and Sperm Parameters in SARS-CoV-2 Patients: A Systematic Review and Meta-Analysis.The world journal of men's health · 2023Article
- Spatial Distribution of SARS-CoV-2 Receptors and Proteases in Testicular Cells.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2023Article
- SARS-CoV-2, fertility and assisted reproduction.Human reproduction update · 2023Review
- SARS-CoV-2 Infection and the Male Reproductive System: A Brief Review.Life (Basel, Switzerland) · 2023Review
- COVID-19 associates with semen inflammation and sperm quality impairment that reverses in the short term after disease recovery.Frontiers in physiology · 2023Article
- Male reproductive health after 3 months from SARS-CoV-2 infection: a multicentric study.Journal of endocrinological investigation · 2023Article
- A review on human reproductive systems encountering with the severe acute respiratory syndrome coronavirus 2 infection.International journal of reproductive biomedicine · 2023Review
- The impact of male factors and their correct and early diagnosis in the infertile couple's pathway: 2021 perspectives.Journal of endocrinological investigation · 2022Review
- COVID-19 Infections in Gonads: Consequences on Fertility?Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme · 2022Review
- Could SARS-CoV-2 infection affect male fertility and sexuality?APMIS : acta pathologica, microbiologica, et immunologica Scandinavica · 2022Review
- Assessment of SARS-CoV-2 RNA shedding in semen of 36 males with symptomatic, asymptomatic, and convalescent infection during the first and second wave of COVID-19 pandemic in Italy.Asian journal of andrologyArticle
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeDue to relevant repercussions on reproductive medicine, we aimed to evaluate feasibility of RT-PCR as a detection method of SARS-CoV-2 RNA in seminal fluid.
methodsA qualitative determination of the RT-PCR assays in semen was performed through different approaches: (1) efficiency of RNA extraction from sperm and seminal plasma was determined using PRM1 and PRM2 mRNA and a heterologous system as control; (2) samples obtained by diluting viral preparation from a SARS-CoV-2 panel (virus cultured in Vero E6 cell lines) were tested; (3) viral presence in different fractions of seminal fluid (whole sample, seminal plasma and post-centrifugation pellet) was evaluated. Semen samples from mild and recovered COVID-19 subjects were collected by patients referring to the Infectious Disease Department of the Policlinico Umberto I Hospital - "Sapienza" University of Rome. Control subjects were recruited at the Laboratory of Seminology-Sperm Bank "Loredana Gandini'' of the same hospital.
resultsThe control panel using viral preparations diluted in saline and seminal fluid showed the capability to detect viral RNA presence with C
conclusionThese preliminary data show that RT-PCR for SARS-CoV-2 RNA testing appears to be a feasible method for the molecular diagnosis of SARS-CoV-2 in seminal fluid, supported by results of the control panel. The ability to detect SARS-CoV-2 in semen is extremely important for reproductive medicine, especially in assisted reproductive technology and sperm cryopreservation.
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Registered trials
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