Evidence map›Paper›PMID 33929101›Full record

ArticleMicrobial biotechnology2021

A multiplex antigen microarray for simultaneous IgG and IgM detection against SARS-CoV-2 reveals higher seroprevalence than reported.

David Ruano-Gallego, Miriam García-Villadangos, Mercedes Moreno-Paz, Javier Gómez-Elvira, Marina Postigo, María Simón-Sacristán, Hugh T Reyburn, Carlo Carolis, Natalia Rodrigo, Yaiza B Codeseira and 4 more

Open access · goldAbstract read
In one paragraph

Article in Microbial biotechnology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
1.0field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 17 citations in OpenAlex.

  1. Review
  2. ACS pharmacology & translational science · 2025
    Article
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  4. Article
  5. Rapid and quantitative detection of multiple antibodies against SARS-CoV-2 mutant proteins by photo-immobilized microarray.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2022
    Article
  6. Article
  7. Article
  8. Article
  9. Enzyme Therapy: Current Challenges and Future Perspectives.International journal of molecular sciences · 2021
    Review
  10. Article
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 6 institutions in 1 country.

David Ruano-GallegoDepartment of Molecular Evolution, Centro de Astrobiología (INTA-CSIC), Carretera de Ajalvir km 4, Torrejón de Ardoz, Madrid, 28850, Spain.ORCID 0000-0002-2163-2088
Miriam García-VilladangosDepartment of Molecular Evolution, Centro de Astrobiología (INTA-CSIC), Carretera de Ajalvir km 4, Torrejón de Ardoz, Madrid, 28850, Spain.ORCID 0000-0002-3928-3592
Mercedes Moreno-PazDepartment of Molecular Evolution, Centro de Astrobiología (INTA-CSIC), Carretera de Ajalvir km 4, Torrejón de Ardoz, Madrid, 28850, Spain.ORCID 0000-0003-1245-3253
Javier Gómez-ElviraSpace Payload Department, Instituto Nacional de Técnica Aeroespacial (INTA), Carretera de Ajalvir km 4, Torrejón de Ardoz, Madrid, 28850, Spain.ORCID 0000-0002-9068-9846
Marina PostigoDepartment of Molecular Evolution, Centro de Astrobiología (INTA-CSIC), Carretera de Ajalvir km 4, Torrejón de Ardoz, Madrid, 28850, Spain.ORCID 0000-0002-4041-3788
María Simón-SacristánMicrobiology and Parasitology Unit, Hospital Central de la Defensa 'Gómez Ulla', Glorieta del Ejército 1, Madrid, 28050, Spain.
Hugh T ReyburnDepartment of Immunology and Oncology, Centro Nacional de Biotecnología (CNB-CSIC), C/ Darwin 3, Madrid, 28049, Spain.ORCID 0000-0003-2855-1595
Carlo CarolisCRG-Centre for Genomic Regulation, Dr. Aiguader 88, Barcelona, 08003, Spain.ORCID 0000-0003-4240-1139
Natalia RodrigoCRG-Centre for Genomic Regulation, Dr. Aiguader 88, Barcelona, 08003, Spain.ORCID 0000-0002-6050-2446
Yaiza B CodeseiraCIMUS Biomedical Research Institute, University of Santiago de Compostela-IDIS, Avenida de Barcelona s/n, Santiago de Compostela, 15782, Spain.ORCID 0000-0001-8120-7262
Paloma RuedaEurofins-INGENASA, Avenida Institución Libre de Enseñanza 41, Madrid, Spain.ORCID 0000-0002-7735-3766
Sonia ZúñigaDepartment of Molecular and Cellular Biology, Centro Nacional de Biotecnología (CNB-CSIC), C/ Darwin 3, Madrid, 28049, Spain.ORCID 0000-0003-2549-6826
Luis EnjuanesDepartment of Molecular and Cellular Biology, Centro Nacional de Biotecnología (CNB-CSIC), C/ Darwin 3, Madrid, 28049, Spain.ORCID 0000-0002-0854-0226
Victor ParroDepartment of Molecular Evolution, Centro de Astrobiología (INTA-CSIC), Carretera de Ajalvir km 4, Torrejón de Ardoz, Madrid, 28850, Spain.ORCID 0000-0003-3738-0724
Centro de Astrobiología · ESCentro Nacional de Biotecnología · ESCentre for Genomic Regulation · ESHospital Central de la Defensa Gómez Ulla · ESInstituto Nacional de Técnica Aeroespacial · ESUniversidade de Santiago de Compostela · ES

Funding

Centro Superior de Investigaciones Cientificas 202020E079Comunidad de Madrid 2019-T2/TIC-15235Instituto Nacional de Técnica AeroespacialINTA's internal budget and the Spanish Ministry of Science and Innovation MDM-2017-0737INTA's internal budget and the Spanish Ministry of Science and Innovation RTI2018-094368-B-I00Ministerio de Ciencia e Innovación MDM-2017-0737Ministerio de Ciencia e Innovación RTI2018-094368-B-I00'Programa de Atracción de Talento' of local Government of Madrid
6 · The paper itself

Abstract

The surge of SARS-CoV-2 has challenged health systems worldwide and efficient tests to detect viral particles, as well as antibodies generated against them, are needed. Specificity, sensitivity, promptness or scalability are the main parameters to estimate the final performance, but rarely all of them match in a single test. We have developed SCOVAM, a protein microarray with several viral antigens (spike, nucleocapsid, main protease Nsp5) as capturing probes in a fluorescence immunoassay for COVID-19 serological testing. SCOVAM depicts IgG and IgM antibody responses against each of these proteins of 22 individuals in a single microscope slide. It detects specific IgM (0.094 μg ml

Indexed as

COVID-19SARS-CoV-2Antibodies, ViralCOVID-19 Serological TestingCOVID-19 SerotherapyHumansImmunization, PassiveImmunoglobulin GImmunoglobulin MSensitivity and SpecificitySeroepidemiologic StudiesSpike Glycoprotein, CoronavirusAntibodies, ViralImmunoglobulin GImmunoglobulin MSpike Glycoprotein, Coronavirus

Identifiers

PMID33929101
PMCPMC8085928
OpenAlexW3157222399

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.