Evidence map›Paper›PMID 33928582›Full record

ReviewMethods in molecular biology (Clifton, N.J.)2021

Fundamental and Practical Aspects in the Formulation of Colloidal Polyelectrolyte Complexes of Chitosan and siRNA.

Christophe Schatz, Tim Delas

Open access · greenAbstract readReview
PubMed Publisher
In one paragraph

Review in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.6field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 2 institutions in 1 country.

Christophe SchatzLaboratoire de Chimie des Polymères Organiques (LCPO), University of Bordeaux, CNRS, Bordeaux INP, UMR 5629, Pessac, France.
Tim DelasLaboratoire de Chimie des Polymères Organiques (LCPO), University of Bordeaux, CNRS, Bordeaux INP, UMR 5629, Pessac, France. timdelas@yahoo.fr.
École Nationale Supérieure de Chimie, de Biologie et de Physique · FRLaboratoire de Chimie des Polymères Organiques · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The formation of electrostatic interactions between polyanionic siRNA and polycations gives an easy access to the formation of colloidal particles capable of delivering siRNA in vitro or in vivo. Among the polycations used for siRNA delivery, chitosan occupies a special place due to its unique physicochemical and biological properties. In this chapter we describe the fundamental and practical aspects of the formation of colloidal complexes between chitosan and siRNA. The basis of the electrostatic complexation between oppositely charged polyelectrolytes is first introduced with a focus on the specific conditions to obtain stable colloid complex particles. Subsequent, the properties that make chitosan so special are described. In a third part, the main parameters influencing the colloidal properties and stability of siRNA/chitosan complexes are reviewed with emphasis on some practical aspects to consider in the preparation of complexes.

Indexed as

RNA InterferenceTransfectionAnimalsCell LineChitosanColloidsHumansPolyelectrolytesRNA, Small InterferingStatic ElectricityChitosanColloidsPolyelectrolytesRNA, Small InterferingBiocolloidsChitosanComplex particlesElectrostatic interactionPolyelectrolyte complexationsiRNA

Identifiers

PMID33928582
OpenAlexW3157284796

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.