Evidence map›Paper›PMID 33928551›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Identification of Human Antigen-Specific CD4+ Cells with Peptide-MHC Multimer Technologies.

I-Ting Chow, William W Kwok

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. CD4+ T Cell Epitope Identification from Complex Parasite Antigen Mixtures.Methods in molecular biology (Clifton, N.J.) · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

I-Ting ChowThe Benaroya Research Institute at Virginia Mason, Seattle, WA, USA.
William W KwokThe Benaroya Research Institute at Virginia Mason, Seattle, WA, USA. bkwok@benaroyaresearch.org.

Funding

Phenotypic Analysis of Islet Antigen-specific Effector T Cells in Pre-diabetic SubjectsDP3DK106909 · NIDDK · BENAROYA RESEARCH INST AT VIRGINIA MASON · PI KWOK, WILLIAM W. · 2015 to 2015
$2.2M
NIDDK NIH HHS DP3 DK106909
6 · The paper itself

Abstract

Peptide-major histocompatibility complex class II (pMHCII) multimers have emerged as a convenient and powerful tool for characterization of CD4 T cell immune responses in a large variety of human diseases. Peptide-MHCII multimers can rapidly identify peptide antigens recognized by CD4 T cells via high-throughput peptide screening procedures. The specificity and phenotype of antigen-specific CD4 T cells can be effectively visualized by pMHCII multimers from unmanipulated immune cell populations. Functional attributes of antigen-specific CD4 T cells can also be defined with the multimer technology in combination with immune functional assays such as intracellular cytokine staining (ICS).

Indexed as

Cell SeparationEpitope MappingEpitopesFlow CytometryCD4-Positive T-LymphocytesCells, CulturedCytokinesHigh-Throughput Screening AssaysHistocompatibility Antigens Class IIHumansPhenotypeProtein MultimerizationResearch DesignWorkflowCytokinesEpitopesHistocompatibility Antigens Class IIAntigen-specific CD4 T cellsEpitopesMHC class II multimers

Identifiers

PMID33928551

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.