Evidence map›Paper›PMID 33928383›Full record

ArticleJournal of animal science2021

Early supplementation with Lactobacillus plantarum in liquid diet modulates intestinal innate immunity through toll-like receptor 4-mediated mitogen-activated protein kinase signaling pathways in young piglets challenged with Escherichia coli K88.

Kuanmin M Yang, Cui Zhu, Li Wang, Shuting T Cao, Xuefen F Yang, Kaiguo G Gao, Zongyong Y Jiang

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of animal science, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.9field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 11 citations in OpenAlex.

  1. Oral administration ofApplied and environmental microbiology · 2026
    Article
  2. The bile acid-gut microbiota-vitamin D axis: new insights into biliary atresia.Frontiers in cellular and infection microbiology · 2026
    Review
  3. Article
  4. Review
  5. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 2 countries.

Kuanmin M YangState Key Laboratory of Livestock and Poultry Breeding, Key Laboratory of Animal Nutrition and Feed in South China, Ministry of Agriculture and Rural Affairs, Guangdong, China. Key Laboratory of Animal Breeding and Nutrition, Maoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Institute of Animal Science, China, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, P.R. China.
Cui ZhuSchool of Life Sciences and Engineering, Foshan University, Foshan 528225, P.R. China.
Li WangState Key Laboratory of Livestock and Poultry Breeding, Key Laboratory of Animal Nutrition and Feed in South China, Ministry of Agriculture and Rural Affairs, Guangdong, China. Key Laboratory of Animal Breeding and Nutrition, Maoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Institute of Animal Science, China, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, P.R. China.ORCID 0000-0001-5084-9596
Shuting T CaoState Key Laboratory of Livestock and Poultry Breeding, Key Laboratory of Animal Nutrition and Feed in South China, Ministry of Agriculture and Rural Affairs, Guangdong, China. Key Laboratory of Animal Breeding and Nutrition, Maoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Institute of Animal Science, China, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, P.R. China.
Xuefen F YangState Key Laboratory of Livestock and Poultry Breeding, Key Laboratory of Animal Nutrition and Feed in South China, Ministry of Agriculture and Rural Affairs, Guangdong, China. Key Laboratory of Animal Breeding and Nutrition, Maoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Institute of Animal Science, China, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, P.R. China.
Kaiguo G GaoState Key Laboratory of Livestock and Poultry Breeding, Key Laboratory of Animal Nutrition and Feed in South China, Ministry of Agriculture and Rural Affairs, Guangdong, China. Key Laboratory of Animal Breeding and Nutrition, Maoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Institute of Animal Science, China, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, P.R. China.
Zongyong Y JiangState Key Laboratory of Livestock and Poultry Breeding, Key Laboratory of Animal Nutrition and Feed in South China, Ministry of Agriculture and Rural Affairs, Guangdong, China. Key Laboratory of Animal Breeding and Nutrition, Maoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Institute of Animal Science, China, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, P.R. China.
Guangdong Academy of Agricultural Sciences · CNFoshan University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study was conducted to investigate the effects of early supplementation during 4 to 18 d of age with Lactobacillus plantarum (LP) in liquid diets on intestinal innate immune response in young piglets infected with enterotoxigenic Escherichia coli (ETEC) K88. Seventy-two barrow piglets at 4 d old were assigned to basal or LP-supplemented liquid diet (5 × 1010 CFU·kg-1). On day 15, piglets from each group were orally challenged with either ETEC K88 (1 × 108 CFU·kg-1) or the same amount of phosphate-buffered saline. The intestinal mucosa, mesenteric lymph node (MLN), and spleen samples were collected on day 18. Here, we found that LP pretreatment significantly decreased the mRNA relative expression of inflammatory cytokines (interleukin [IL]-1β, IL-8, and tumor necrosis factor-α), porcine β-defensin 2 (pBD-2), and mucins (MUC1 and MUC4) in the jejunal mucosa in piglets challenged with ETEC K88 (P < 0.05). Moreover, LP significantly decreased the ileal mucosa mRNA relative expression of IL-8 and MUC4 in young piglets challenged with ETEC K88 (P < 0.05). Furthermore, the piglets of the LP + ETEC K88 group had lower protein levels of IL-8, secretory immunoglobulin A, pBD-2, and MUC4 in the jejunal mucosa than those challenged with ETEC K88 (P < 0.05). Besides, LP supplementation reduced the percentage of gamma/delta T cells receptor (γδTCR) and CD172a+ (SWC3+) cells in MLN and the percentage of γδTCR cells in the spleen of young piglets after the ETEC K88 challenge. Supplementation with LP in liquid diets prevented the upregulated protein abundance of toll-like receptor (TLR) 4, phosphorylation-p38, and phosphorylation-extracellular signal-regulated protein kinases in the jejunal mucosa induced by ETEC K88 (P < 0.05). In conclusion, LP supplementation in liquid diet possesses anti-inflammatory activity and modulates the intestinal innate immunity during the early life of young piglets challenged with ETEC K88, which might be attributed to the suppression of TLR4-mediated mitogen-activated protein kinase signaling pathways. Early supplementation with LP in liquid diets regulates the innate immune response, representing a promising immunoregulation strategy for maintaining intestinal health in weaned piglets.

Indexed as

Enterotoxigenic Escherichia coliEscherichia coli InfectionsLactiplantibacillus plantarumAnimalsDietDietary SupplementsImmunity, InnateIntestinal MucosaMitogen-Activated Protein KinasesSwineToll-Like Receptor 4Mitogen-Activated Protein KinasesToll-Like Receptor 4enterotoxigenic Escherichia coli K88intestinal innate immunityLactobacillus plantarummitogen-activated protein kinasepigletstoll-like receptor 4

Identifiers

PMID33928383
PMCPMC8212570
OpenAlexW3157353277

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.