ArticleOptica2020
Hyperspectral multiphoton microscopy for
Article in Optica, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 31 citations in OpenAlex.
- Phasor-Based Spatio-Spectral Segmentation for Hyperspectral Fluorescence Microscopy.Journal of biophotonics · 2026Article
- Deep-Learning-Driven High-Fidelity In Vivo Hyperspectral Fluorescence Imaging Under Extreme Photon-Limited Conditions.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Multiplexed video hyperspectral microendoscopy reveals tumor-immune dynamics in vivo.Research square · 2026Article
- High-throughput hyperspectral fluorescence imaging using a high-speed silicon photomultiplier array.Biomedical optics express · 2025Article
- hyperPICASSO: adapting the mutual-information-based unmixing technique PICASSO to hyperspectral datasets.Optics letters · 2025Article
- Supercontinuum-tailoring multicolor imaging reveals spatiotemporal dynamics of heterogeneous tumor evolution.Nature communications · 2024Article
- BINGO: a blind unmixing algorithm for ultra-multiplexing fluorescence images.Bioinformatics (Oxford, England) · 2024Article
- SpeCamX: mobile app that turns unmodified smartphones into multispectral imagers.Biomedical optics express · 2023Article
- Multi-objective optimization of custom compound prism arrays for multiplexed optical imaging.Optics express · 2023Article
- Intravital imaging to study cancer progression and metastasis.Nature reviews. Cancer · 2023Review
- Denoising multiplexed microscopy images in n-dimensional spectral space.Biomedical optics express · 2022Article
- Microscopy is better in color: development of a streamlined spectral light path for real-time multiplex fluorescence microscopy.Biomedical optics express · 2022Article
- Trends in hyperspectral imaging: from environmental and health sensing to structure-property and nano-bio interaction studies.Analytical and bioanalytical chemistry · 2022Article
- In vivo simultaneous nonlinear absorption Raman and fluorescence (SNARF) imaging of mouse brain cortical structures.Communications biology · 2022Article
- Multiphoton intravital microscopy of rodents.Nature reviews. Methods primers · 2022Article
- Article
- Two-photon peak molecular brightness spectra reveal long-wavelength enhancements of multiplexed imaging depth and photostability.Biomedical optics express · 2021Article
- Synchronously pumped Raman laser for simultaneous degenerate and nondegenerate two-photon microscopy.Biomedical optics express · 2021Article
- DMD-based hyperspectral microscopy with flexible multiline parallel scanning.Microsystems & nanoengineering · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 2 institutions in 1 country.
Funding
Abstract
The insensitivity of multiphoton microscopy to optical scattering enables high-resolution, high-contrast imaging deep into tissue, including in live animals. Scattering does, however, severely limit the use of spectral dispersion techniques to improve spectral resolution. In practice, this limited spectral resolution together with the need for multiple excitation wavelengths to excite different fluorophores limits multiphoton microscopy to imaging a few, spectrally-distinct fluorescent labels at a time, restricting the complexity of biological processes that can be studied. Here, we demonstrate a hyperspectral multiphoton microscope that utilizes three different wavelength excitation sources together with multiplexed fluorescence emission detection using angle-tuned bandpass filters. This microscope maintains scattering insensitivity, while providing high enough spectral resolution on the emitted fluorescence and capitalizing on the wavelength-dependent nonlinear excitation of fluorescent dyes to enable clean separation of multiple, spectrally overlapping labels,
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.