Evidence map›Paper›PMID 33927995›Full record

Article3 Biotech2021

Generation of biologically active recombinant human OCT4 protein from

Chandrima Dey, Madhuri Thool, Srirupa Bhattacharyya, S Sudhagar, Rajkumar P Thummer

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in 3 Biotech, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.0field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 12 citations in OpenAlex.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Chandrima Dey *Laboratory for Stem Cell Engineering and Regenerative Medicine, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, 781039 Assam India.
Madhuri Thool *Laboratory for Stem Cell Engineering and Regenerative Medicine, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, 781039 Assam India.
Srirupa BhattacharyyaDepartment of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, 781039 Assam India.
S SudhagarDepartment of Biotechnology, National Institute of Pharmaceutical Education and Research Guwahati, Changsari, 781101 Guwahati, Assam India.
Rajkumar P ThummerLaboratory for Stem Cell Engineering and Regenerative Medicine, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, 781039 Assam India.ORCID 0000-0002-4526-8364
Indian Institute of Technology Guwahati · INNational Institute of Pharmaceutical Education and Research · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Octamer-binding transcription factor 4 (OCT4) is vital for early embryonic development and is a master regulator of pluripotency in embryonic stem cells. Notably, OCT4 is a key reprogramming factor to derive induced pluripotent stem cells, which have tremendous prospects in regenerative medicine. In the current study, we report heterologous expression and purification of human OCT4 in SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s13205-021-02758-z.

Indexed as

E. coliOCT4Protein expressionProtein purificationRecombinant proteinSecondary structure

Identifiers

PMID33927995
PMCPMC8032842
OpenAlexW3149932564

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.