ArticleScientific reports2021
Estrogen withdrawal alters cytoskeletal and primary ciliary dynamics resulting in increased Hedgehog and osteoclastogenic paracrine signalling in osteocytes.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 28 citations in OpenAlex.
- A Modular Bioinstructive Platform Reveals Mechanistic Insights into Additive-Free, Topography-Driven Osteogenesis.Advanced healthcare materials · 2026Article
- Targeting integrin αVβ3-Ptgs2-mTOR signaling rescues bone formation in osteoporosis: from molecular mechanism toward therapy.Cellular & molecular biology letters · 2026Article
- The role and mechanisms of bone microenvironment regulators in osteoporosis: novel intervention strategies for addressing the challenges of aging.Frontiers in endocrinology · 2026Review
- Ciliary-Mediated Mechanotransduction in Skeletal Development and Diseases.Results and problems in cell differentiation · 2026Review
- The interplay of estrogen, gut microbiome, and bone immunity in osteoporosis.Cell communication and signaling : CCS · 2025Review
- The Role of Primary Cilia in Modulating the Luteinization Process of Ovarian Granulosa Cells in Mice.International journal of molecular sciences · 2025Article
- In Vitro Assay to Examine Osteoclast Resorptive Activity Under Estrogen Withdrawal.Bio-protocol · 2025Article
- The Nociceptor Primary Cilium Contributes to Mechanical Nociceptive Threshold and Inflammatory and Neuropathic Pain.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Article
- Review
- Myopic shift in female mice after ovariectomy.Scientific reports · 2024Article
- Altered extracellular matrix and mechanotransduction gene expression in rat bone tissue following long-term estrogen deficiency.JBMR plus · 2024Article
- In Vitro Cell Culture Model for Osteoclast Activation during Estrogen Withdrawal.International journal of molecular sciences · 2024Article
- Osteocytes and Primary Cilia.Current osteoporosis reports · 2023Review
- The High Level of RANKL Improves IκB/p65/Cyclin D1 Expression and Decreases p-Stat5 Expression in Firm Udder of Dairy Goats.International journal of molecular sciences · 2023Article
- Recent advances in primary cilia in bone metabolism.Frontiers in endocrinology · 2023Review
- Network pharmacology of iridoid glycosides from Eucommia ulmoides Oliver against osteoporosis.Scientific reports · 2022Article
- Cytoskeletal Protein 4.1G Is Essential for the Primary Ciliogenesis and Osteoblast Differentiation in Bone Formation.International journal of molecular sciences · 2022Article
- The Impact of the Extracellular Matrix Environment onBioengineering (Basel, Switzerland) · 2022Article
- Osteocytes and Estrogen Deficiency.Current osteoporosis reports · 2021Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Estrogen deficiency during post-menopausal osteoporosis leads to osteoclastogenesis and bone loss. Increased pro-osteoclastogenic signalling (RANKL/OPG) by osteocytes occurs following estrogen withdrawal (EW) and is associated with impaired focal adhesions (FAs) and a disrupted actin cytoskeleton. RANKL production is mediated by Hedgehog signalling in osteocytes, a signalling pathway associated with the primary cilium, and the ciliary structure is tightly coupled to the cytoskeleton. Therefore, the objective of this study was to investigate the role of the cilium and associated signalling in EW-mediated osteoclastogenic signalling in osteocytes. We report that EW leads to an elongation of the cilium and increase in Hedgehog and osteoclastogenic signalling. Significant trends were identified linking cilia elongation with reductions in cell area and % FA area/cell area, indicating that cilia elongation is associated with disruption of FAs and actin contractility. To verify this, we inhibited FA assembly via α
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