Evidence map›Paper›PMID 33925983›Full record

ReviewNanomaterials (Basel, Switzerland)2021

Microfluidics for Peptidomics, Proteomics, and Cell Analysis.

Rui Vitorino, Sofia Guedes, João Pinto da Costa, Václav Kašička

Abstract readReview
In one paragraph

Review in Nanomaterials (Basel, Switzerland), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed.

  1. Review
  2. Review
  3. Review
  4. Review
  5. Review
  6. Review
  7. Article
  8. Article
  9. Current Challenges and Future Directions in Peptidomics.Methods in molecular biology (Clifton, N.J.) · 2024
    Article
  10. Review
  11. Review
  12. Review
  13. Review
  14. Review
  15. Peptidomics.Nature reviews. Methods primers · 2023
    Article
  16. Review
  17. Review
  18. Article
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Rui VitorinoUnIC, Departamento de Cirurgia e Fisiologia, Faculdade de Medicina da Universidade do Porto, 4785-999 Porto, Portugal.ORCID 0000-0003-3636-5805
Sofia GuedesLAQV/REQUIMTE, Department of Chemistry, University of Aveiro, 00351234 Aveiro, Portugal.
João Pinto da CostaDepartment of Chemistry & Center for Environmental and Marine Studies (CESAM), University of Aveiro, 00351234 Aveiro, Portugal.ORCID 0000-0002-1871-7160
Václav KašičkaInstitute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, Flemigovo n. 542/2, 166 10 Prague 6, Czech Republic.

Funding

Fundação para a Ciência e a Tecnologia 1223
6 · The paper itself

Abstract

Microfluidics is the advanced microtechnology of fluid manipulation in channels with at least one dimension in the range of 1-100 microns. Microfluidic technology offers a growing number of tools for manipulating small volumes of fluid to control chemical, biological, and physical processes relevant to separation, analysis, and detection. Currently, microfluidic devices play an important role in many biological, chemical, physical, biotechnological and engineering applications. There are numerous ways to fabricate the necessary microchannels and integrate them into microfluidic platforms. In peptidomics and proteomics, microfluidics is often used in combination with mass spectrometric (MS) analysis. This review provides an overview of using microfluidic systems for peptidomics, proteomics and cell analysis. The application of microfluidics in combination with MS detection and other novel techniques to answer clinical questions is also discussed in the context of disease diagnosis and therapy. Recent developments and applications of capillary and microchip (electro)separation methods in proteomic and peptidomic analysis are summarized. The state of the art of microchip platforms for cell sorting and single-cell analysis is also discussed. Advances in detection methods are reported, and new applications in proteomics and peptidomics, quality control of peptide and protein pharmaceuticals, analysis of proteins and peptides in biomatrices and determination of their physicochemical parameters are highlighted.

Indexed as

cell sortingLOCmicrochip electrophoresismicrofluidicsmicroTASpeptidespeptidomicsproteinsproteomicssingle-cell analysis

Identifiers

PMID33925983
PMCPMC8145566

What OpenQuestion holds

Textmetadata
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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.