ArticleInternational journal of molecular sciences2021
Isolation of Small Extracellular Vesicles from Human Sera.
Article in International journal of molecular sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Wedelactone-loaded exosomes for sepsis-induced liver injury: a novel therapeutic strategy.Drug delivery · 2026Article
- Plant-Derived Nanovesicles: A Comprehensive Review from Isolation to Clinical Translation-Unlocking Natural Nanocarriers for Biomedical Applications.Biomolecules · 2026Review
- Lipid Messengers: Mechanisms and Clinical Applications of Exosomal Lipids in Neurodegenerative Diseases.Molecular neurobiology · 2026Review
- Fibroblast-derived small extracellular vesicles loaded with tacrolimus enhances dermal delivery and alleviates cytokine-overdriven skin inflammation.Drug delivery and translational research · 2026Article
- Human Blood Exosomes: Isolation and Characterization Methods, Variability, and the Need for Standardized Protocols-A Review.Biomedicines · 2025Review
- Cell Membrane- and Vesicle-Based Bionic Nanodrugs: Applications in Central Nervous System Diseases and Exploration of Nasal-Cerebral Delivery.Gels (Basel, Switzerland) · 2025Review
- ICP-MS for Multiplexed Protein Determination in Extracellular Vesicles from APP/PS1 Mice Blood Serum-Application to a Zn Supplementation Pilot Study.Analytical chemistry · 2025Article
- Morphological Characterization of Plasma-Derived Nanoparticles Isolated by High-Speed Ultracentrifugation: A Scanning Electron Microscopy Study.International journal of molecular sciences · 2025Article
- Tuberculosis Patients' Serum Extracellular Vesicles Induce Relevant Immune Responses for Initial Defense Against BCG in Mice.Microorganisms · 2025Article
- Head and neck squamous cell carcinoma-derived extracellular vesicles mediate Ca²⁺-dependent platelet activation and aggregation through tissue factor.Cell communication and signaling : CCS · 2025Article
- The Secretome of the Inductive Tooth Germ Exhibits Signals Required for Tooth Development.Bioengineering (Basel, Switzerland) · 2025Article
- Article
- Label-Free, Sensitive, and Versatile Colorimetric Method for Molecule Detection via the G-Quadruplex-Based Signal Quenching Strategy.ACS omega · 2024Article
- Thermophoretic glycan profiling of extracellular vesicles for triple-negative breast cancer management.Nature communications · 2024Article
- Small extracellular vesicles are released ex vivo from platelets into serum and from residual blood cells into stored plasma.Journal of extracellular biology · 2023Article
- A review on comparative studies addressing exosome isolation methods from body fluids.Analytical and bioanalytical chemistry · 2023Review
- Release of exosomes in polytraumatized patients: The injury pattern is reflected by the surface epitopes.Frontiers in immunology · 2023Article
- Microvesicle-camouflaged biomimetic nanoparticles encapsulating a metal-organic framework for targeted rheumatoid arthritis therapy.Journal of nanobiotechnology · 2022Article
- Methods for the Detection of Circulating Biomarkers in Cancer Patients.Advances in experimental medicine and biology · 2022Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Robust, well-characterized methods for purifying small extracellular vesicles (sEV) from blood are needed before their potential as disease biomarkers can be realized. Here, we compared isolation of sEV from serum by differential ultracentrifugation (DUC) and by exclusion chromatography using commercially available Exo-spin™ columns. We show that sEV can be purified by both methods but Exo-spin™ columns contain copious additional particles recorded by nanoparticle tracking analysis, invalidating its use for quantifying yields. DUC samples contained higher concentrations of exosome specific proteins CD9, CD63 and CD81 and electron microscopy confirmed that most particles in DUC preparations were sEV, whereas Exo-spin™ samples also contained copious co-purified plasma lipids. MACSPlex bead analysis identified multiple exosome surface proteins, with stronger signals in DUC samples, enabling detection of 21 of 37, compared to only 10 in Exo-spin™ samples. Nevertheless, the pattern of expression was consistent in both preparations, indicating that lipids do not interfere with bead-based technologies. Thus, both DUC and Exo-spin™ can be used to isolate sEV from human serum and what is most appropriate depends on the subsequent use of sEV. In summary, Exo-spin™ enables isolation of sEV from blood with vesicle populations similar to the ones recovered by DUC, but with lower concentrations.
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