ArticleInternational journal of molecular sciences2021
The Effects of Cryogenic Storage on Human Dental Pulp Stem Cells.
Article in International journal of molecular sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 27 citations in OpenAlex.
- Phenotyping of Human Pulp Cells After Cryopreservation.Journal of cellular and molecular medicine · 2026Article
- Clinical Potential of Long-Term Cryopreserved Stem Cells From Impacted Third Molar Pulps for Regenerative Endodontic Procedures: An Ex Vivo Evaluation.Australian endodontic journal : the journal of the Australian Society of Endodontology Inc · 2026Article
- Evaluation of Deep Eutectic Solvents for Cryopreservation of the Fish PathogenMethods and protocols · 2026Article
- Viability of Dental Pulp Derived Stem Cells After Long-Term Cryopreservation.Journal of endodontics · 2025Article
- New considerations in selecting donors for dental pulp stem cells: a pilot study.Biomedical engineering online · 2025Article
- Effects of simulated microgravity on dental pulp stem cell stemness.Journal of molecular histology · 2025Article
- Intrinsic and extrinsic modulators of human dental pulp stem cells: advancing strategies for tissue engineering applications.Molecular biology reports · 2025Review
- A transcriptomic analysis of dental pulp stem cell senescence in vitro.Biomedical engineering online · 2024Article
- Decellularized human amniotic membrane scaffolds: influence on the biological behavior of dental pulp stem cells.BMC oral health · 2024Article
- How to make full use of dental pulp stem cells: an optimized cell culture method based on explant technology.Frontiers in bioengineering and biotechnology · 2024Article
- Research progress on optimization ofFrontiers in bioengineering and biotechnology · 2024Review
- Improved Method for Dental Pulp Stem Cell Preservation and Its Underlying Cell Biological Mechanism.Cells · 2023Article
- Cultivation of Cryopreserved Human Dental Pulp Stem Cells-A New Approach to Maintaining Dental Pulp Tissue.International journal of molecular sciences · 2022Article
- Strategies to Convert Cells into Hyaline Cartilage: Magic Spells for Adult Stem Cells.International journal of molecular sciences · 2022Review
- Article
- Stem Cells from Dental Sources: Translational Applications in Medicine and Novel Approaches.International journal of molecular sciences · 2022Article
- Clinical Potential of Dental Pulp Stem Cells in Pulp Regeneration: Current Endodontic Progress and Future Perspectives.Frontiers in cell and developmental biology · 2022Review
- Dental Pulp Stem Cells Derived From Adult Human Third Molar Tooth: A Brief Review.Frontiers in cell and developmental biology · 2021Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Dental pulp stem cells (DPSCs) are a type of easily accessible adult mesenchymal stem cell. Due to their ease of access, DPSCs show great promise in regenerative medicine. However, the tooth extractions from which DPSCs can be obtained are usually performed at a period of life when donors would have no therapeutic need of them. For this reason, it is imperative that successful stem cell storage techniques are employed so that these cells remain viable for future use. Any such techniques must result in high post-thaw stem cell recovery without compromising stemness, proliferation, or multipotency. Uncontrolled-rate freezing is not a technically or financially demanding technique compared to expensive and laborious controlled-rate freezing techniques. This study was aimed at observing the effect of uncontrolled-rate freezing on DPSCs stored for 6 and 12 months. Dimethyl sulfoxide at a concentration of 10% was used as a cryoprotective agent. Various features such as shape, proliferation capacity, phenotype, and multipotency were studied after DPSC thawing. The DPSCs did not compromise their stemness, viability, proliferation, or differentiating capabilities, even after one year of cryopreservation at -80 °C. After thawing, they retained their stemness markers and low-level expression of hematopoietic markers. We observed a size reduction in recovery DPSCs after one year of storage. This observation indicates that DPSCs can be successfully used in potential clinical applications, even after a year of uncontrolled cryopreservation.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.