Evidence map›Paper›PMID 33922553›Full record

ArticleMicromachines2021

An Integrated Centrifugal Degassed PDMS-Based Microfluidic Device for Serial Dilution.

Anyang Wang, Samaneh Moghadasi Boroujeni, Philip J Schneider, Liam B Christie, Kyle A Mancuso, Stelios T Andreadis, Kwang W Oh

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Micromachines, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.6field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 11 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Anyang WangSensors and MicroActuators Learning Lab (SMALL), Department of Electrical Engineering, The State University of New York at Buffalo, Buffalo, NY 14260, USA.ORCID 0000-0003-1897-8915
Samaneh Moghadasi BoroujeniDepartment of Chemical and Biological Engineering, University at Buffalo, State University of New York (SUNY-Buffalo), Buffalo, NY 14260, USA.
Philip J SchneiderSensors and MicroActuators Learning Lab (SMALL), Department of Electrical Engineering, The State University of New York at Buffalo, Buffalo, NY 14260, USA.
Liam B ChristieSensors and MicroActuators Learning Lab (SMALL), Department of Electrical Engineering, The State University of New York at Buffalo, Buffalo, NY 14260, USA.
Kyle A MancusoSensors and MicroActuators Learning Lab (SMALL), Department of Electrical Engineering, The State University of New York at Buffalo, Buffalo, NY 14260, USA.
Stelios T AndreadisDepartment of Chemical and Biological Engineering, University at Buffalo, State University of New York (SUNY-Buffalo), Buffalo, NY 14260, USA.
Kwang W OhSensors and MicroActuators Learning Lab (SMALL), Department of Electrical Engineering, The State University of New York at Buffalo, Buffalo, NY 14260, USA.ORCID 0000-0003-0078-6683
Sensors (United States) · USUniversity at Buffalo, State University of New York · US

Funding

Direct reprogramming of epidermal cells to neural crest derivatives for cell therapiesR01EB023114 · NIBIB · STATE UNIVERSITY OF NEW YORK AT BUFFALO · PI ANDREADIS, STELIOS THEOHARIS · 2016 to 2019
$1.7M
Mark Diamond Research Fund of the Graduate Student Association at the University at Buffalo, the State University of New York N/ANIBIB NIH HHS R01 EB023114Qualcomm Faculty Award N/A
6 · The paper itself

Abstract

We propose an integrated serial dilution generator utilizing centrifugal force with a degassed polydimethylsiloxane (PDMS) microfluidic device. Using gas-soluble PDMS as a centrifugal microfluidic device material, the sample can be dragged in any arbitrary direction using vacuum-driven force, as opposed to in a single direction, without adding further actuation components. The vacuum-driven force allows the device to avoid the formation of air bubbles and exhibit high tolerance in the surface condition. The device was then used for sample metering and sample transferring. In addition, centrifugal force was used for sample loading and sample mixing. In this study, a series of ten-fold serial dilutions ranging from 100 to 10-4 with about 8 μL in each chamber was achieved, while the serial dilution ratio and chamber volume could easily be altered by changing the geometrical designs of the device. As a proof of concept of our hybrid approach with the centrifugal and vacuum-driven forces, ten-fold serial dilutions of a cDNA (complementary DNA) sample were prepared using the device. Then, the diluted samples were collected by fine needles and subject to a quantitative polymerase chain reaction (qPCR), and the results were found to be in good agreement with those for samples prepared by manual pipetting.

Indexed as

centrifugal pumpingmicrofluidicPDMSqPCRserial dilutionvacuum pumping

Identifiers

PMID33922553
PMCPMC8145514
OpenAlexW3157691765

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.