ArticleBiology2021
Impact of Implant Surface Material and Microscale Roughness on the Initial Attachment and Proliferation of Primary Human Gingival Fibroblasts.
Article in Biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 45 citations in OpenAlex.
- Cellular Responses at the Zirconia Dental Implant Interface: A Comprehensive Review.Journal of functional biomaterials · 2026Review
- Evaluation of surface roughness and cell viability of milled and printed polyetheretherketone healing abutments: a comparative study.Odontology · 2026Article
- Biomimetic Surface Engineering of Ti-15Zr (Roxolid™) Implants: Enhancing Osseointegration and Bone Regeneration-A Comprehensive Review.Biomimetics (Basel, Switzerland) · 2026Review
- Impact of Zirconia and Titanium Implant Surfaces of Different Roughness on Oral Epithelial Cells.Dentistry journal · 2026Article
- Soft tissue integration around dental implants: A pressing priority.Biomaterials · 2026Review
- Polycatecholamine nanocoatings on stainless steel: the effect on attachment of human fibroblasts and platelets.Beilstein journal of nanotechnology · 2026Article
- Biomolecule-functionalized dental implant surfaces: Towards augmenting soft tissue integration.Bioactive materials · 2025Review
- Impact of Atomic Layer-Deposited Hydroxyapatite-Coated Titanium on Expression of Focal Adhesion Molecules of Human Gingival Fibroblasts.Nanomaterials (Basel, Switzerland) · 2025Article
- A comparative study of the epithelial regeneration capacities of two biomaterials in vitro.BMC oral health · 2025Article
- TNF-α Activates NF-κB Signalling Pathway in MG-63 Cells on Titanium and Zirconia Implant Surfaces.Materials (Basel, Switzerland) · 2025Article
- Optimization of PETG 3D printing parameters for the design and development of biocompatible bone implants.Frontiers in bioengineering and biotechnology · 2025Article
- Architectural Design of Biomaterial Scaffolds for Hematopoietic Stem Cell Culture.Biomaterials research · 2025Review
- Wound Closure Promotion by Leucine-Based Pseudo-Proteins: An In Vitro Study.International journal of molecular sciences · 2024Article
- Roughness affects the response of human fibroblasts and macrophages to sandblasted abutments.Biomedical engineering online · 2024Article
- Comparison of Zirconia Implant Surface Modifications for Optimal Osseointegration.Journal of functional biomaterials · 2024Review
- Piranha-etched titanium nanostructure reduces biofilm formation in vitro.Clinical oral investigations · 2023Article
- Effect of Bergenin on Human Gingival Fibroblast Response on Zirconia Implant Surfaces: An In Vitro Study.Journal of functional biomaterials · 2023Article
- Leucine-Based Pseudo-Proteins (LPPs) as Promising Biomaterials: A Study of Cell-Supporting Properties.Polymers · 2023Article
- Guiding Fibroblast Activation Using an RGD-Mutated Heparin Binding II Fragment of Fibronectin for Gingival Titanium Integration.Advanced healthcare materials · 2023Article
- Surface Coatings of Dental Implants: A Review.Journal of functional biomaterials · 2023Review
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
Abstract
Due to the rising demand for zirconia (Zr) based implant systems, it is important to understand the impact of Zr and titanium (Ti) implants and particularly their topography on soft tissue healing. As human gingival fibroblasts (hGFs) are the predominant cells in peri-implant soft tissue, we focused on examining the effect of implant material and surface roughness on hGFs' initial attachment, growth and the expression of proteins involved in the focal adhesion. hGFs isolated from eight healthy donors were cultured on the following surfaces: smooth titanium machined surface (TiM), smooth zirconia machined surface (ZrM), moderately rough titanium surface (SLA), or moderately rough zirconia surface (ZLA) for up to 14 days. The initial attachment of hGFs was evaluated by scanning electron microscopy. Cell proliferation/viability was assessed by cell counting kit 8. Focal adhesion and cytoskeleton were visualized by a focal adhesion staining kit. The gene expression of focal adhesion kinase (FAK), α-smooth muscle actin (α-SMA), and integrin subunits ITG-β1, ITG-β4, ITG-α4, ITG-α5, ITG-α6, was evaluated by qPCR. Cell proliferation/viability was slightly decreased by moderately rough surfaces, whereas no effect of surface material was observed. Cell morphology was strikingly different between differently treated surfaces: on machined surfaces, cells had elongated morphology and were attached along the grooves, whereas on moderately rough surfaces, cells were randomly attached. Surface roughness had a more pronounced effect on the gene expression compared to the surface material. The expression of FAK, α-SMA, ITG-β4, ITG-α5, and ITG-α6 was enhanced by moderately rough surfaces compared to smooth surfaces. Within the limitations of this in vitro study, it can be concluded that the behavior of primary hGFs is primarily affected by surface structure, whereas no apparent advantage of Zr over Ti could be observed.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.