Evidence map›Paper›PMID 33921330›Full record

ArticleSensors (Basel, Switzerland)2021

Immobilized Antibodies on Mercaptophenylboronic Acid Monolayers for Dual-Strategy Detection of 20S Proteasome.

Madalina M Barsan, Caroline G Sanz, Melania Onea, Victor C Diculescu

Open access · goldAbstract read
In one paragraph

Article in Sensors (Basel, Switzerland), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 6 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Madalina M BarsanNational Institute of Materials Physics, Atomistilor 405A, 077125 Măgurele, Romania.
Caroline G SanzNational Institute of Materials Physics, Atomistilor 405A, 077125 Măgurele, Romania.ORCID 0000-0002-2829-7163
Melania OneaNational Institute of Materials Physics, Atomistilor 405A, 077125 Măgurele, Romania.
Victor C DiculescuNational Institute of Materials Physics, Atomistilor 405A, 077125 Măgurele, Romania.
National Institute of Materials Physics · ROUniversity of Bucharest · RO

Funding

Ministerul Cercetării şi Inovării Core Program PN19-030101 (contract no.21/2019)Ministerul Cercetării şi Inovării Operational Programme Competitiveness 2014-2020, Project: NANOBIOSURF-SMIS 103528
6 · The paper itself

Abstract

A dual strategy for the electrochemical detection for 20S proteasome (20S) is proposed, based on the oriented immobilization of a capture monoclonal antibody (Abβ) on a self-assembled monolayer of 4-mercaptophenylboronic acid (4-MPBA) on gold electrodes, which led to the Au/4-MPBA/Abβ immunosensor. The methodology comprises the correlation of 20S concentration with (i) its proteolytic activity toward the Z-LLE-AMC substrate, using the Au/4-MPBA/Abβ/20S, and (ii) the enzymatic activity of an alkaline phosphatase (AlkP) from the AlkP-labeled secondary antibody (Ab

Indexed as

Antibodies, ImmobilizedBiosensing TechniquesElectrodesGoldImmunoassayProteasome Endopeptidase ComplexAntibodies, ImmobilizedGoldProteasome Endopeptidase Complex20S proteasomealkaline phosphataseelectrochemistryenzymatic activityimmunosensorlabeled antibodymercaptophenylboronic acidoriented antibodiesproteolysisself-assembled monolayers

Identifiers

PMID33921330
PMCPMC8068791
OpenAlexW3155178540

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.