Evidence map›Paper›PMID 33920669›Full record

ArticlePharmaceuticals (Basel, Switzerland)2021

The Comparison of In Vitro Photosensitizing Efficacy of Curcumin-Loaded Liposomes Following Photodynamic Therapy on Melanoma MUG-Mel2, Squamous Cell Carcinoma SCC-25, and Normal Keratinocyte HaCaT Cells.

Marta Woźniak, Martyna Nowak, Anastasiia Lazebna, Kamil Więcek, Izabella Jabłońska, Krzysztof Szpadel, Aleksandra Grzeszczak, Jerzy Gubernator, Piotr Ziółkowski

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Pharmaceuticals (Basel, Switzerland), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed, 1 pooled it
6.0field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed, 1 synthesis or guideline pooled it, 50 citations in OpenAlex.

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  13. Evaluation of the Antitumor Activity of Quaternary Ammonium Surfactants.International journal of molecular sciences · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Marta WoźniakDepartment of Pathology, Wroclaw Medical University, 50-367 Wroclaw, Poland.ORCID 0000-0001-7978-6330
Martyna NowakDepartment of Pathology, Wroclaw Medical University, 50-367 Wroclaw, Poland.
Anastasiia LazebnaDepartment of Biotechnology, University of Wroclaw, 50-383 Wroclaw, Poland.
Kamil WięcekDepartment of Biotechnology, University of Wroclaw, 50-383 Wroclaw, Poland.
Izabella JabłońskaDepartment of Pathology, Wroclaw Medical University, 50-367 Wroclaw, Poland.ORCID 0000-0002-8189-344X
Krzysztof SzpadelDepartment of Pathology, Wroclaw Medical University, 50-367 Wroclaw, Poland.ORCID 0000-0002-6625-157X
Aleksandra GrzeszczakDepartment of Lipids and Liposomes, Faculty of Biotechnology, University of Wroclaw, 50-383 Wroclaw, Poland.
Jerzy GubernatorDepartment of Lipids and Liposomes, Faculty of Biotechnology, University of Wroclaw, 50-383 Wroclaw, Poland.
Piotr ZiółkowskiDepartment of Pathology, Wroclaw Medical University, 50-367 Wroclaw, Poland.
Wroclaw Medical University · PLUniversity of Wrocław · PL

Funding

Wroclaw Medical University SUB.A010.20.13.
6 · The paper itself

Abstract

The research focused on the investigation of curcumin encapsulated in hydrogenated soy phosphatidylcholine liposomes and its increased photoactive properties in photodynamic therapy (PDT). The goal of this study was two-fold: to emphasize the role of a natural photoactive plant-based derivative in the liposomal formulation as an easily bioavailable, alternative photosensitizer (PS) for the use in PDT of skin malignancies. Furthermore, the goal includes to prove the decreased cytotoxicity of phototoxic agents loaded in liposomes toward normal skin cells. Research was conducted on melanoma (MugMel2), squamous cell carcinoma (SCC-25), and normal human keratinocytes (HaCaT) cell lines. The assessment of viability with MTT (3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide) evaluated cell death after exposure to blue light irradiation after 4 h of pre-incubation with free and encapsulated curcumin. Additionally, the wound healing assay, flow cytometry, and immunocytochemistry to detect apoptosis were performed. The malignant cells revealed increased phototoxicity after the therapy in comparison to normal cells. Moreover, liposome curcumin-based photodynamic therapy showed an increased ratio of apoptotic and necrotic cells. The study also demonstrated that nanocurcumin significantly decreased malignant cell motility following PDT treatment. Acquired results suggest that liposomal formulation of a poor soluble natural compound may improve photosensitizing properties of curcumin-mediated PDT treatment in skin cancers and reduce toxicity in normal keratinocytes.

Indexed as

curcuminliposomesmelanomanatural photosensitizernormal keratinocytesphotodynamic therapyskin cancer treatmentsquamous cell carcinoma

Identifiers

PMID33920669
PMCPMC8072566
OpenAlexW3153465818

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.