Evidence map›Paper›PMID 33918210›Full record

ArticleCells2021

Impaired Mitochondrial Function in iPSC-Retinal Pigment Epithelium with the Complement Factor H Polymorphism for Age-Related Macular Degeneration.

Mara C Ebeling, Zhaohui Geng, Rebecca J Kapphahn, Heidi Roehrich, Sandra R Montezuma, James R Dutton, Deborah A Ferrington

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cells, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.

0numbers the graph read from it
0cells of the map it votes in
35citing papers in PubMed
6.7field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

35 citing papers in PubMed, 46 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Mara C EbelingDepartment of Ophthalmology and Visual Neurosciences, University of Minnesota, Minneapolis, MN 55455, USA.
Zhaohui GengStem Cell Institute, University of Minnesota, Minneapolis, MN 55455, USA.
Rebecca J KapphahnDepartment of Ophthalmology and Visual Neurosciences, University of Minnesota, Minneapolis, MN 55455, USA.
Heidi RoehrichHistology Core for Vision Research, University of Minnesota, Minneapolis, MN 55455, USA.ORCID 0000-0002-2232-9494
Sandra R MontezumaDepartment of Ophthalmology and Visual Neurosciences, University of Minnesota, Minneapolis, MN 55455, USA.ORCID 0000-0003-3731-8082
James R DuttonStem Cell Institute, University of Minnesota, Minneapolis, MN 55455, USA.ORCID 0000-0002-5074-2614
Deborah A FerringtonDepartment of Ophthalmology and Visual Neurosciences, University of Minnesota, Minneapolis, MN 55455, USA.
University of Minnesota · US

Funding

Mitochondrial defects in the retinal pigment epithelium and the CFH risk allele for age-related macular degenerationR01EY028554 · NEI · UNIVERSITY OF MINNESOTA · PI DUTTON, JAMES R., FERRINGTON, DEBORAH ANN · 2018 to 2025
$4.5M
Age-related macular degeneration,mitochondria and the CFH genotypeR01EY026012 · NEI · UNIVERSITY OF MINNESOTA · PI FERRINGTON, DEBORAH ANN · 2016 to 2019
$1.7M
NIH HHS P30EY11374NIH HHS R01EY0285541
6 · The paper itself

Abstract

Age-related macular degeneration (AMD), the leading cause of vision loss in the elderly, is characterized by loss of the retinal pigment epithelium (RPE). While the disease mechanism remains unclear, prior studies have linked AMD with RPE mitochondrial defects and genetic polymorphisms in the complement pathway. This study used RPE generated from induced pluripotent stem cells (iPSC-RPE), which were derived from human donors with or without AMD and genotyped for the complement factor H (CFH) AMD high-risk allele (rs1061170, Y402H) to investigate whether donor disease state or genotype had a detrimental effect on mitochondrial function and inflammation. Results show that cells derived from donors with AMD display decreased mitochondrial function under conditions of stress and elevated expression of inflammatory markers compared to iPSC-RPE from individuals without AMD. A more pronounced reduction in mitochondrial function and increased inflammatory markers was observed in CFH high-risk cells, irrespective of disease state. These results provide evidence for a previously unrecognized link between CFH and mitochondrial function that could contribute to RPE loss in AMD patients harboring the CFH high-risk genotype.

Indexed as

Polymorphism, GeneticAgedAged, 80 and overBiomarkersCell LineComplement Factor HComplement System ProteinsEpithelial CellsFemaleHumansInduced Pluripotent Stem CellsInflammationMacular DegenerationMaleMiddle AgedMitochondriaBiomarkersComplement Factor HComplement System ProteinsMitochondrial Proteinsage-related macular degenerationcomplement factor Hinduced pluripotent stem cellinflammationmitochondrial functionretinal pigment epithelium

Identifiers

PMID33918210
PMCPMC8066149
OpenAlexW3147418878

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.