ArticleMicroorganisms2021
Probe-Based Real-Time qPCR Assays for a Reliable Differentiation of Capripox Virus Species.
Article in Microorganisms, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Emerging Goatpox Virus Threat in Wild Ruminants: First Documented Outbreak in the United Arab Emirates, 2024.Veterinary sciences · 2026Article
- Article
- Characterization and molecular identification of the lumpy skin disease virus in cattle in the Mekong Delta of Vietnam.Open veterinary journal · 2024Article
- Unveiling the secrets of adeno-associated virus: novel high-throughput approaches for the quantification of multiple serotypes.Molecular therapy. Methods & clinical development · 2023Article
- A Real-Time Recombinase Polymerase Amplification Assay for Specific Detection of Lumpy Skin Disease Virus.Veterinary sciences · 2023Article
- Cross-Protection of an Inactivated and a Live-Attenuated Lumpy Skin Disease Virus Vaccine against Sheeppox Virus Infections in Sheep.Vaccines · 2023Article
- Article
- A novel triplex real-time PCR assay for the differentiation of lumpy skin disease virus, goatpox virus, and sheeppox virus.Frontiers in veterinary science · 2023Article
- Characterization of a Nigerian Lumpy Skin Disease Virus Isolate after Experimental Infection of Cattle.Pathogens (Basel, Switzerland) · 2021Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Outbreaks of the three capripox virus species, namely lumpy skin disease virus, sheeppox virus, and goatpox virus, severely affect animal health and both national and international economies. Therefore, the World Organization for Animal Health (OIE) classified them as notifiable diseases. Until now, discrimination of capripox virus species was possible by using different conventional PCR protocols. However, more sophisticated probe-based real-time qPCR systems addressing this issue are, to our knowledge, still missing. In the present study, we developed several duplex qPCR assays consisting of different types of fluorescence-labelled probes that are highly sensitive and show a high analytical specificity. Finally, our assays were combined with already published diagnostic methods to a diagnostic workflow that enables time-saving, reliable, and robust detection, differentiation, and characterization of capripox virus isolates.
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