Evidence map›Paper›PMID 33917139›Full record

ArticleViruses2021

Full-Genome Sequences and Phylogenetic Analysis of Archived Danish European Bat Lyssavirus 1 (EBLV-1) Emphasize a Higher Genetic Resolution and Spatial Segregation for Sublineage 1a.

Sten Calvelage, Conrad M Freuling, Anthony R Fooks, Dirk Höper, Denise A Marston, Lorraine McElhinney, Thomas Bruun Rasmussen, Stefan Finke, Martin Beer, Thomas Müller

Open access · goldAbstract read
In one paragraph

Article in Viruses, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.3field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 10 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 3 countries.

Sten CalvelageInstitute of Diagnostic Virology, Friedrich-Loeffler-Institut (FLI), 17493 Greifswald-Insel Riems, Germany.ORCID 0000-0001-8511-9067
Conrad M FreulingCentral Duties, Friedrich-Loeffler-Institut (FLI), 17493 Greifswald-Insel Riems, Germany.ORCID 0000-0002-1076-398X
Anthony R FooksAnimal & Plant Health Agency, WHO Collaborating Centre for Rabies Surveillance and Research, OIE Reference Laboratory for Rabies, Weybridge KT15 3NB, Surrey, UK.ORCID 0000-0002-3243-6154
Dirk HöperInstitute of Diagnostic Virology, Friedrich-Loeffler-Institut (FLI), 17493 Greifswald-Insel Riems, Germany.ORCID 0000-0001-8408-2274
Denise A MarstonAnimal & Plant Health Agency, WHO Collaborating Centre for Rabies Surveillance and Research, OIE Reference Laboratory for Rabies, Weybridge KT15 3NB, Surrey, UK.ORCID 0000-0001-9215-088X
Lorraine McElhinneyAnimal & Plant Health Agency, WHO Collaborating Centre for Rabies Surveillance and Research, OIE Reference Laboratory for Rabies, Weybridge KT15 3NB, Surrey, UK.ORCID 0000-0002-6022-348X
Thomas Bruun RasmussenVirus & Mikrobiologisk Specialdiagnostik, Statens Serum Institut, 2300 Copenhagen, Denmark.
Stefan FinkeInstitute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut (FLI), WHO Collaborating Centre for Rabies Surveillance and Research, OIE Reference Laboratory for Rabies, 17493 Greifswald-Insel Riems, Germany.ORCID 0000-0001-8716-2341
Martin BeerInstitute of Diagnostic Virology, Friedrich-Loeffler-Institut (FLI), 17493 Greifswald-Insel Riems, Germany.
Thomas MüllerInstitute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut (FLI), WHO Collaborating Centre for Rabies Surveillance and Research, OIE Reference Laboratory for Rabies, 17493 Greifswald-Insel Riems, Germany.ORCID 0000-0002-0959-3653
Friedrich-Loeffler-Institut · DEAnimal and Plant Health Agency · GBStatens Serum Institut · DK

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

European bat lyssavirus type 1 (EBLV-1) is the causative agent for almost all reported rabies cases found in European bats. In recent years, increasing numbers of available EBLV-1 full genomes and their phylogenetic analyses helped to further elucidate the distribution and genetic characteristics of EBLV-1 and its two subtypes, namely EBLV-1a and EBLV-1b. Nonetheless, the absence of full-genome sequences from regions with known detections of EBLV-1 still limit the understanding of the phylogeographic relations between viruses from different European regions. In this study, a set of 21 archived Danish EBLV-1 samples from the years 1985 to 2009 was processed for the acquisition of full-genome sequences using a high-throughput sequencing approach. Subsequent phylogenetic analysis encompassing all available EBLV-1 full genomes from databases revealed the Danish sequences belong to the EBLV-1a subtype and further highlighted the distinct, close phylogenetic relationship of Danish, Dutch and German isolates in this region. In addition, the formation of five putative groups nearly exclusively formed by Danish isolates and the overall increased resolution of the EBLV-1a branch indicate a higher genetic diversity and spatial segregation for this sublineage than was previously known. These results emphasize the importance of phylogenetic analyses of full-genome sequences of lyssaviruses for genetic geography.

Indexed as

Genome, ViralPhylogenyAnimalsArchivesChiropteraChromosome MappingChromosome SegregationDenmarkHigh-Throughput Nucleotide SequencingLyssavirusRabiesWhole Genome SequencingbatsEuropean bat lyssavirus 1 (EBLV-1)full-genome sequencingnext generation sequencing (NGS)zoonoses

Identifiers

PMID33917139
PMCPMC8067844
OpenAlexW3142178874

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.