ArticleMicromachines2021
Numerical Simulation and Experimental Verification of Droplet Generation in Microfluidic Digital PCR Chip.
Article in Micromachines, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
5 citing papers in PubMed.
- Article
- CFD study and experimental verification of droplet formation characteristics in a flow-focusing microfluidic device.Biotechnology notes (Amsterdam, Netherlands) · 2025Article
- Microdroplet PCR in Microfluidic Chip Based on Constant Pressure Regulation.Micromachines · 2023Article
- Image Segmentation and Quantification of Droplet dPCR Based on Thermal Bubble Printing Technology.Sensors (Basel, Switzerland) · 2022Article
- Micro-injection Molded Droplet Generation System for Digital PCR Application.Biochip journal · 2022Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
The generation of droplets is one of the most critical steps in the droplet digital polymerase chain reaction (ddPCR) procedure. In this study, the mechanism of droplet formation in microchannel structure and factors affecting droplet formation were studied. The physical field of laminar two-phase flow level was used to simulate the process of droplet generation through microfluidic technology. The effect of the parameters including flow rate, surface tension, and viscosity on the generated droplet size were evaluated by the simulation. After that, the microfluidic chip that has the same dimension as the simulation was then, fabricated and evaluated. The chip was made by conventional SU-8 photolithography and injection molding. The accuracy of the simulation was validated by comparing the generated droplets in the real scenario with the simulation result. The relative error (RE) between experimentally measured droplet diameter and simulation results under different flow rate, viscosity, surface tension and contact angle was found less than 3.5%, 1.8%, 1.4%, and 1.2%, respectively. Besides, the coefficient of variation (CV) of the droplet diameter was less than 1%, which indicates the experimental droplet generation was of high stability and reliability. This study provides not only fundamental information for the design and experiment of droplet generation by microfluidic technology but also a reliable and efficient investigation method in the ddPCR field.
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Registered trials
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