Evidence map›Paper›PMID 33916868›Full record

ArticleInternational journal of molecular sciences2021

Uptake of MicroRNAs from Exosome-Like Nanovesicles of Edible Plant Juice by Rat Enterocytes.

Yuko Ito, Kohei Taniguchi, Yuki Kuranaga, Nabil Eid, Yosuke Inomata, Sang-Woong Lee, Kazuhisa Uchiyama

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
38citing papers in PubMed, 1 pooled it
3.3field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

38 citing papers in PubMed, 1 synthesis or guideline pooled it, 66 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 2 countries.

Yuko ItoDepartment of General and Gastrological Surgery, Osaka Medical College, 2-7, Takatsuki, Osaka 569-8686, Japan.ORCID 0000-0002-1983-3745
Kohei TaniguchiDepartment of General and Gastrological Surgery, Osaka Medical College, 2-7, Takatsuki, Osaka 569-8686, Japan.ORCID 0000-0003-0648-1370
Yuki KuranagaUnited Graduate School of Drug Discovery and Medical Information Sciences, Gifu University, 1-1, Yanagito, Gifu 501-1193, Japan.ORCID 0000-0001-7418-6520
Nabil EidDepartment of Anatomy, College of Medicine and Health Sciences, United Arab Emirates University, P.O. Box 17666 Al Ain, United Arab Emirates.ORCID 0000-0002-2938-2618
Yosuke InomataDepartment of General and Gastrological Surgery, Osaka Medical College, 2-7, Takatsuki, Osaka 569-8686, Japan.ORCID 0000-0003-4897-5707
Sang-Woong LeeDepartment of General and Gastrological Surgery, Osaka Medical College, 2-7, Takatsuki, Osaka 569-8686, Japan.
Kazuhisa UchiyamaDepartment of General and Gastrological Surgery, Osaka Medical College, 2-7, Takatsuki, Osaka 569-8686, Japan.
Osaka Medical and Pharmaceutical University · JPGifu University · JPUnited Arab Emirates University · AE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

MicroRNAs (miRNAs) are small RNAs present in extracellular vesicles (EVs) that, when transferred to a target cell, affect its biological functions. Plant miRNAs regulate the expression of certain mammalian genes. Here, we characterized EVs in fruit and vegetable juice, and their miRNA cargo, and investigated whether such miRNA-containing EVs could be taken up by mammalian enterocytes in vitro. Using filtration and ultra-centrifugation methods, EVs were purified from commercially available and manually squeezed plant juice. EV morphological features and subcellular localization were analyzed using the NanoSight tracking system and electron microscopy. Plant EV miRNA levels were evaluated using quantitative reverse transcription PCR. For the in vitro EV uptake experiments, rat intestinal epithelial cells (IEC6) were used. Plant EVs shared morphological features with mammalian EVs and contained miR156a-5p, miR166a-3p, and miR168a-5p. EVs were present in the cell sap-filled central vacuoles and were taken up by IEC6 cells. Edible plant cells produce EVs that contain various miRNAs and release them into the central vacuole. The exogenous plant EVs are taken up by mammalian enterocytes in vitro. These findings suggest the possibility that exogenous plant miRNAs carried by EVs can be absorbed via the gastrointestinal tract.

Indexed as

Fruit and Vegetable JuicesMicroRNAsNanoparticlesAnimalsCell LineEnterocytesExosomesRatsMicroRNAsedible plantenterocyteexosomeextracellular vesiclemicroRNA

Identifiers

PMID33916868
PMCPMC8038500
OpenAlexW3142099809

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.