Evidence map›Paper›PMID 33916128›Full record

ArticleLife (Basel, Switzerland)2021

Multipotent Stromal Cell Extracellular Vesicle Distribution in Distant Organs after Introduction into a Bone Tissue Defect of a Limb.

Igor Maiborodin, Aleksandr Shevela, Michael Toder, Sergey Marchukov, Natalya Tursunova, Marina Klinnikova, Vitalina Maiborodina, Elena Lushnikova, Andrew Shevela

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Life (Basel, Switzerland), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 48% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 1 country.

Igor MaiborodinInstitute of Molecular Pathology and Pathomorphology, Federal State Budget Scientific Institution "Federal Research Center of Fundamental and Translational Medicine", Ministry of Science and Higher Education of the Russian Federation, Akademika Timakova st., 2, 630117 Novosibirsk, Russia.
Aleksandr ShevelaThe Center of New Medical Technologies, Institute of Chemical Biology and Fundamental Medicine, The Russian Academy of Sciences, Siberian Branch, Akademika Lavrenteva str., 8, 630090 Novosibirsk, Russia.
Michael ToderInternational Center of Dental Implantology "iDent", Sibrevkoma st., 9b, 630007 Novosibirsk, Russia.
Sergey MarchukovThe Center of New Medical Technologies, Institute of Chemical Biology and Fundamental Medicine, The Russian Academy of Sciences, Siberian Branch, Akademika Lavrenteva str., 8, 630090 Novosibirsk, Russia.
Natalya TursunovaInstitute of Molecular Pathology and Pathomorphology, Federal State Budget Scientific Institution "Federal Research Center of Fundamental and Translational Medicine", Ministry of Science and Higher Education of the Russian Federation, Akademika Timakova st., 2, 630117 Novosibirsk, Russia.
Marina KlinnikovaInstitute of Molecular Pathology and Pathomorphology, Federal State Budget Scientific Institution "Federal Research Center of Fundamental and Translational Medicine", Ministry of Science and Higher Education of the Russian Federation, Akademika Timakova st., 2, 630117 Novosibirsk, Russia.
Vitalina MaiborodinaInstitute of Molecular Pathology and Pathomorphology, Federal State Budget Scientific Institution "Federal Research Center of Fundamental and Translational Medicine", Ministry of Science and Higher Education of the Russian Federation, Akademika Timakova st., 2, 630117 Novosibirsk, Russia.
Elena LushnikovaInstitute of Molecular Pathology and Pathomorphology, Federal State Budget Scientific Institution "Federal Research Center of Fundamental and Translational Medicine", Ministry of Science and Higher Education of the Russian Federation, Akademika Timakova st., 2, 630117 Novosibirsk, Russia.
Andrew ShevelaThe Center of New Medical Technologies, Institute of Chemical Biology and Fundamental Medicine, The Russian Academy of Sciences, Siberian Branch, Akademika Lavrenteva str., 8, 630090 Novosibirsk, Russia.
Siberian Branch of the Russian Academy of Sciences · RUFederal Research Center of Fundamental and Translational Medicine · RUInstitute of Molecular Pathology and Pathomorphology · RUInstitute of Chemical Biology and Fundamental Medicine · RU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

When administered intravenously, extracellular vesicles derived from multipotent stromal cells (MSC EVs) immediately pass through the lungs along with the blood and regularly spread to all organs. When administered intraperitoneally, they are absorbed either into the blood or into the lymph and are quickly disseminated throughout the body. The possibility of generalized spread of MSC EVs to distant organs in case of local intratissular administration remains unexplored. However, it is impossible to exclude MSC EV influence on tissues distant from the injection site due to the active or passive migration of these injected nanoparticles through the vessels. The research is based on findings obtained when studying the samples of lungs, heart, spleen, and liver of outbred rabbits of both sexes weighing 3-4 kg at various times after the injection of EVs derived from MSCs of bone marrow origin and labeled by PKH26 into an artificially created defect of the proximal condyle of the tibia. MSC EVs were isolated by serial ultracentrifugation and characterized by transmission electron microscopy and flow cytometry. After the introduction of MSC EVs into the damaged proximal condyle of the tibia of rabbits, these MSC EVs can be found most frequently in the lungs, myocardium, liver, and spleen. MSC EVs enter all of these organs with the blood flow. The lungs contained the maximum number of labeled MSC EVs; moreover, they were often associated with detritus and were located in the lumen of the alveoli. In the capillary network of various organs except the myocardium, MSC EVs are adsorbed by paravasal phagocytes; in some cases, specifically labeled small dust-like objects can be detected throughout the entire experiment-up to ten days of observation. Therefore, we can conclude that the entire body, including distant organs, is effected both by antigenic detritus, which appeared in the bloodstream after extensive surgery, and MSC EVs introduced from the outside.

Indexed as

bone tissue defect of a limbexosomesextracellular vesicles distributionheartliverlungsmultipotent stromal cell extracellular vesiclesspleen

Identifiers

PMID33916128
PMCPMC8066794
OpenAlexW3141786835

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.