ArticleViruses2021
Updated and Validated Pan-Coronavirus PCR Assay to Detect All Coronavirus Genera.
Article in Viruses, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 38 citations in OpenAlex.
- DivergentViruses · 2026Article
- Surveillance of Swine Coronaviruses in Hungarian Herds with a Newly Established Pan-Coronavirus RT-PCR System.Animals : an open access journal from MDPI · 2026Article
- First report on detection and molecular characterization of alphacoronaviruses in the small Indian mongoose (Urva auropunctata).Virology journal · 2025Article
- Article
- Correction: Holbrook et al. Updated and Validated Pan-Coronavirus PCR Assay to Detect All Coronavirus Genera.Viruses · 2025Article
- Discovery, phylogenetic, and comparative genomic analysis of novel avian gammacoronaviruses identified in feral pigeons (Journal of virology · 2025Article
- Misidentification of Human Coronavirus HKU1 as OC43 by the Seegene Allplex Respiratory Panel Real-time PCR Assay.Annals of laboratory medicine · 2025Article
- Novel paramyxovirus in wild pinnipeds, Brazil.Veterinary research communications · 2025Article
- Comparative Analysis of Quan and Watanabe Pan-Coronavirus Assays for Bat Coronavirus Diversity in Sarawak, East Malaysia.Journal of medical virology · 2025Article
- Identification and Characterization of Viral and Bacterial Pathogens in Free-Living Bats of Kopaonik National Park, Serbia.Veterinary sciences · 2025Article
- The final frontier: using carcasses for one health surveillance at the ecosystem interface.Frontiers in veterinary science · 2025Article
- Article
- Article
- First Detection of Gammacoronavirus in a Striped Dolphin (Animals : an open access journal from MDPI · 2024Article
- Exploring the Potential ofViruses · 2024Article
- Hibernating vesper bats are a weak source for biomonitoring of coronaviruses.One health (Amsterdam, Netherlands) · 2024Article
- Comparative Performance in the Detection of Four Coronavirus Genera from Human, Animal, and Environmental Specimens.Viruses · 2024Article
- Survey of white-footed mice (Peromyscus leucopus) in Connecticut, USA reveals low SARS-CoV-2 seroprevalence and infection with divergent betacoronaviruses.Npj viruses · 2023Article
- Genomic screening of 16 UK native bat species through conservationist networks uncovers coronaviruses with zoonotic potential.Nature communications · 2023Article
- Genetic diversity among reptilian orthoreoviruses isolated from pet snakes and lizards.Frontiers in veterinary science · 2023Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Coronavirus (CoV) spillover events from wildlife reservoirs can result in mild to severe human respiratory illness. These spillover events underlie the importance of detecting known and novel CoVs circulating in reservoir host species and determining CoV prevalence and distribution, allowing improved prediction of spillover events or where a human-reservoir interface should be closely monitored. To increase the likelihood of detecting all circulating genera and strains, we have modified primers published by Watanabe et al. in 2010 to generate a semi-nested pan-CoV PCR assay. Representatives from the four coronavirus genera (α-CoVs, β-CoVs, γ-CoVs and δ-CoVs) were tested and all of the in-house CoVs were detected using this assay. After comparing both assays, we found that the updated assay reliably detected viruses in all genera of CoVs with high sensitivity, whereas the sensitivity of the original assay was lower. Our updated PCR assay is an important tool to detect, monitor and track CoVs to enhance viral surveillance in reservoir hosts.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.