ArticlePloS one2021
Effects of exogenous ascorbic acid on seed germination and seedling salt-tolerance of alfalfa.
Article in PloS one, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 41 citations in OpenAlex.
- Ascorbic and salicylic acid regulate antioxidant defense and drought stress responses in maize.Plant signaling & behavior · 2026Article
- Tissue- and Condition-Specific Biosynthesis of Ascorbic Acid inInternational journal of molecular sciences · 2025Article
- Biorefinery of sunflower by-products: Optimization of twin-screw extrusion for novel biostimulants.Heliyon · 2025Article
- Analysis on Salinity Tolerance of Lettuce Cultivars Under Saline Irrigation and Application of Organic Acids.Plants (Basel, Switzerland) · 2025Article
- Priming effect of ascorbic acid on the growth and biomass of quinoa under saline conditions.Frontiers in plant science · 2025Article
- Changes in Endogenous Carotenoids, Flavonoids, and Phenolics of Drought-Stressed Broccoli Seedlings After Ascorbic Acid Preconditioning.Plants (Basel, Switzerland) · 2024Article
- Bridging gaps and seeding futures: A synthesis of soil salinization and the role of plant-soil interactions under climate change.iScience · 2024Review
- Application of Exogenous Ascorbic Acid Enhances Cold Tolerance in Tomato Seedlings through Molecular and Physiological Responses.International journal of molecular sciences · 2024Article
- Exogenous ascorbic acid as a potent regulator of antioxidants, osmo-protectants, and lipid peroxidation in pea under salt stress.BMC plant biology · 2024Article
- Dehydrin CaDHN2 Enhances Drought Tolerance by Affecting Ascorbic Acid Synthesis under Drought in Peppers.Plants (Basel, Switzerland) · 2023Article
- Molecular dynamics of seed priming at the crossroads between basic and applied research.Plant cell reports · 2023Review
- Article
- Effects of Ascorbic Acid on Physiological Characteristics during Somatic Embryogenesis ofInternational journal of molecular sciences · 2022Article
- Transcriptome-Wide Analysis Revealed the Potential of the High-Affinity Potassium Transporter (Bioengineering (Basel, Switzerland) · 2022Article
- Metabolome profiling of stratified seeds provides insight into the regulation of dormancy inPlant diversity · 2022Article
- Ascorbic Acid Preconditioning Effect on Broccoli Seedling Growth and Photosynthesis under Drought Stress.Plants (Basel, Switzerland) · 2022Article
- Dynamic Responses of Antioxidant and Glyoxalase Systems to Seed Aging Based on Full-Length Transcriptome in Oat (Antioxidants (Basel, Switzerland) · 2022Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Alfalfa (Medicago sativa L.) is an important legume crop for forage, agriculture, and environment in the world. Ascorbic acid (AsA) plays positive roles in plants. However, its effects on germination and salt-tolerance of alfalfa are unknown. The effects of AsA applications on seed germination and seedling salt-tolerance of alfalfa were investigated. The results revealed that 0.1 and 1 mmol L-1 of exogenous AsA increased germination, amylase, and protease, as well as seedling length, fresh weight (FW), dry weight (DW), and endogenous AsA both in the shoots and roots, except that 1 mmol L-1 AsA reduced the activities of α-amylase, β-amylase and protease on day 3. However, 10 and 100 mmol L-1 AsA inhibited these parameters and even caused serious rot. It indicates that 0.1 mmol L-1 AsA has the optimal effects, whereas 100 mmol L-1 AsA has the worst impacts. Another part of the results showed that 0.1 mmol L-1 AsA not only enhanced stem elongation, FW and DW, but also increased chlorophyll and carotenoids both under non-stress and 150 mmol L-1 NaCl stress. Furthermore, 0.1 mmol L-1 AsA mitigated the damages of membrane permeability, malondialdehyde, and excessive reactive oxygen species (ROS) and ions both in the shoots and roots under 150 mmol L-1 NaCl stress. Hence, 0.1 mmol L-1 AsA improves growth and induces salt-tolerance by inhibiting excessive ROS, down-regulating the ion toxicity and up-regulating the antioxidant system. The principal component analysis included two main components both in the shoots and roots, and it explained the results well. In summary, the optimum concentration of 0.1 mmol L-1 AsA can be implemented to improve the seed germination and seedling growth of alfalfa under salt stress.
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