ArticlePloS one2021
Comparative transcriptome analysis reveals key genes potentially related to organic acid and sugar accumulation in loquat.
Article in PloS one, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 20 citations in OpenAlex.
- Comparative Transcriptomic Analysis Reveals Sugar Metabolism and Accumulation Mechanisms inLife (Basel, Switzerland) · 2026Article
- Foliar Application of MgO Nanoparticles Modulates Magnesium Nutrition and Fruit Quality in Loquat Under Mg-Deficient Conditions.Plants (Basel, Switzerland) · 2026Article
- Transcriptomic Profiling Uncovers Molecular Basis for Sugar and Acid Metabolism in Two Pomegranate (Foods (Basel, Switzerland) · 2025Article
- Multidimensional Transcriptomics Reveals the Key Genes and Pathways Regulating the Acidity of Apples.Current issues in molecular biology · 2025Article
- Identification of key gene networks controlling organic acid and sugar metabolism during star fruit (Averrhoa carambola) development.BMC plant biology · 2024Article
- Characterization of indigenousPeerJ · 2024Article
- Transcriptome differential expression analysis of defoliation in different lemon varieties under drought treatment.PloS one · 2024Article
- Genome-Wide Identification ofInternational journal of molecular sciences · 2023Article
- Postharvest Biology and Technology of Loquat (Foods (Basel, Switzerland) · 2023Review
- Organic Acid Accumulation and Associated Dynamic Changes in Enzyme Activity and Gene Expression during Fruit Development and Ripening of Common Loquat and Its Interspecific Hybrid.Foods (Basel, Switzerland) · 2023Article
- Combined Metabolome and Transcriptome Analyses Unveil the Molecular Mechanisms of Fruit Acidity Variation in Litchi (International journal of molecular sciences · 2023Article
- Sugar and acid profile of loquat (Frontiers in plant science · 2022Article
- An Updated Review on the Modulation of Carbon Partitioning and Allocation in Arbuscular Mycorrhizal Plants.Microorganisms · 2021Review
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Authors and funding
7 authors at 3 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Organic acids and sugars are the primary components that determine the quality and flavor of loquat fruits. In the present study, major organic acids, sugar content, enzyme activities, and the expression of related genes were analyzed during fruit development in two loquat cultivars, 'JieFangZhong' (JFZ) and 'BaiLi' (BL). Our results showed that the sugar content increased during fruit development in the two cultivars; however, the organic acid content dramatically decreased in the later stages of fruit development. The differences in organic acid and sugar content between the two cultivars primarily occured in the late stage of fruit development and the related enzymes showed dynamic changes in activies during development. Phosphoenolpyruvate carboxylase (PEPC) and mNAD malic dehydrogenase (mNAD-MDH) showed higher activities in JFZ at 95 days after flowering (DAF) than in BL. However, NADP-dependent malic enzyme (NADP-ME) activity was the lowest at 95 DAF in both JFZ and BL with BL showing higher activity compared with JFZ. At 125 DAF, the activity of fructokinase (FRK) was significantly higher in JFZ than in BL. The activity of sucrose synthase (SUSY) in the sucrose cleavage direction (SS-C) was low at early stages of fruit development and increased at 125 DAF. SS-C activity was higher in JFZ than in BL. vAI and sucrose phosphate synthase (SPS) activities were similar in the two both cultivars and increased with fruit development. RNA-sequencing was performed to determine the candidate genes for organic acid and sugar metabolism. Our results showed that the differentially expressed genes (DEGs) with the greated fold changes in the later stages of fruit development between the two cultivars were phosphoenolpyruvate carboxylase 2 (PEPC2), mNAD-malate dehydrogenase (mNAD-MDH), cytosolic NADP-ME (cyNADP-ME2), aluminum-activated malate transporter (ALMT9), subunit A of vacuolar H+-ATPase (VHA-A), vacuolar H+-PPase (VHP1), NAD-sorbitol dehydrogenase (NAD-SDH), fructokinase (FK), sucrose synthase in sucrose cleavage (SS-C), sucrose-phosphate synthase 1 (SPS1), neutral invertase (NI), and vacuolar acid invertase (vAI). The expression of 12 key DEGs was validated by quantitative reverese transcription PCR (RT-qPCR). Our findings will help understand the molecular mechanism of organic acid and sugar formation in loquat, which will aid in breeding high-quality loquat cultivars.
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