Evidence map›Paper›PMID 33912845›Full record

ArticleSTAR protocols2021

Detecting single cell interferon-beta production using a fluorescent reporter telomerase-immortalized human fibroblast cell line.

David N Hare, Minomi K Subapanditha, Karen L Mossman

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 95% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

David N HareHealth Sciences, McMaster University, Hamilton, ON L8S 4L8, Canada.
Minomi K SubapandithaBiochemistry and Biomedical Sciences, McMaster University, Hamilton, ON L8S 4L8, Canada.
Karen L MossmanHealth Sciences, McMaster University, Hamilton, ON L8S 4L8, Canada.
McMaster University · CA

Funding

CIHR MOP 57669
6 · The paper itself

Abstract

Recent data suggest that cells respond to infection by upregulating the antiviral cytokine interferon-beta (IFN-ß) in a fraction of infected cells. Approaches are thus needed to study these responses on a single-cell level rather than bulk population. Here, we describe a protocol to analyze the IFN-ß response of individual cells using flow cytometry and immunofluorescence microscopy. We show the heterogeneous IFN-ß response to inactivated Sendai virus and human cytomegalovirus, but this protocol can be adapted to other viruses. For complete details on the use and execution of this protocol, please refer to Hare et al. (2020).

Indexed as

Interferon-betaCell LineFibroblastsFluorescent DyesHumansSingle-Cell AnalysisSingle Molecule ImagingTelomeraseFluorescent DyesInterferon-betaTelomeraseCell-based AssaysCell BiologyFlow Cytometry/Mass CytometryImmunologyMicroscopySingle Cell

Identifiers

PMID33912845
PMCPMC8063907
OpenAlexW3153673529

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.