ArticleiScience2021
Effects of fixation on bacterial cellular dimensions and integrity.
Article in iScience, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed, 30 citations in OpenAlex.
- Click-based determination of accumulation of molecules in Escherichia coli.Nature communications · 2026Article
- Synthetic lipopeptides that interact with lipopolysaccharides are potent bactericidal compounds againstApplied and environmental microbiology · 2025Article
- Integrating taxonomic and phenotypic information through FISH-enhanced flow cytometry for microbial community dynamics analysis.Microbiology spectrum · 2025Article
- Imaging flow cytometry as a novel approach for the diagnosis of heparin-induced thrombocytopenia.British journal of haematology · 2025Article
- Super-Resolution Microscopy of the Bacterial Cell Wall Labeled by Fluorescent D-Amino Acids.Methods in molecular biology (Clifton, N.J.) · 2024Article
- The cell wall lipoprotein CD1687 acts as a DNA binding protein during deoxycholate-induced biofilm formation in Clostridioides difficile.NPJ biofilms and microbiomes · 2023Article
- Expansion Microscopy of Bacillus subtilis.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Opportunities in optical and electrical single-cell technologies to study microbial ecosystems.Frontiers in microbiology · 2023Review
- Human umbilical cord blood cells suffer major modification by fixatives and anticoagulants.Frontiers in physiology · 2023Article
- Outer Membrane Vesicles: Biogenesis, Functions, and Issues.Microbiology and molecular biology reviews : MMBR · 2022Review
- Preparation and Structural Evaluation of Epithelial Cell Monolayers in a Physiologically Sized Microfluidic Culture Device.Journal of visualized experiments : JoVE · 2022Article
- Combining multiple fluorescence imaging techniques in biology: when one microscope is not enough.Molecular biology of the cell · 2022Article
- Spectroscopic investigation of faeces with surface-enhanced Raman scattering: a case study with coeliac patients on gluten-free diet.Analytical and bioanalytical chemistry · 2022Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Fixation facilitates imaging of subcellular localization and cell morphology, yet it remains unknown how fixation affects cellular dimensions and intracellular fluorescence patterns, particularly during long-term storage. Here, we characterized the effects of multiple fixatives on several bacterial species. Fixation generally reduced cell length by 5-15%; single-cell tracking in microfluidics revealed that the length decrease was an aggregate effect of many steps in the fixation protocol and that fluorescence of cytoplasmic GFP but not membrane-bound MreB-msfGFP was rapidly lost with formaldehyde-based fixatives. Cellular dimensions were preserved in formaldehyde-based fixatives for ≥4 days, but methanol caused length to decrease. Although methanol preserved cytoplasmic fluorescence better than formaldehyde-based fixatives, some
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.