ArticleNature protocols2021
Microtiter plate assays to assess antibiofilm activity against bacteria.
Article in Nature protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 122 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
122 citing papers in PubMed.
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- Phosphonium-based disinfectants are as effective as best-in-class quaternary ammonium compounds in the eradication of established biofilms in antibiotic-resistant bacteria.Microbiology spectrum · 2026Article
- MOF on HOF NanoFilms for Efficient Bacteria Infected Wound Healing.Advanced healthcare materials · 2026Article
- Mechanochemical Synthesis of a TiOJournal of functional biomaterials · 2026Article
- Multidimensional Characterization of Surface Properties and Microbial Biofilm Formation in Commercial Flowable Resin-Based Dental Composites.Journal of functional biomaterials · 2026Article
- Transcriptomic Responses of MRSA to Penicillin-Modulating Phlorotannins Derived fromMarine drugs · 2026Article
- Pediococcus acidilactici JUFB as a probiotic antibiofilm adjunct against Enterococcus faecalis in persistent endodontic infections.Antonie van Leeuwenhoek · 2026Article
- Strain-level heterogeneity in Vibrio parahaemolyticus limits the predictive value of baseline planktonic gene expression for surface-associated phenotypes.Scientific reports · 2026Article
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- Inter-Crystal Spacing of Implantable Polymeric Surfaces as a Key Suppressor of Microbial Adhesion.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Antibiofilm efficacy and genomic characterization of a lytic bacteriophage targeting MDR uropathogenic Escherichia coli.Archives of microbiology · 2026Article
- Ethosomal nanocarrier encapsulating curcumin for antimicrobial photodynamic therapy: efficacy analysis against mature Streptococcus mutans biofilm.BMC oral health · 2026Article
- GK-11: a Novel Cationic Peptide with Antibiofilm Activity against Staphylococcus aureus and Pseudomonas aeruginosa.Probiotics and antimicrobial proteins · 2026Article
- Selenium nanoparticles functionalized with bacitracin: a novel approach for combating multidrug-resistant bacteria and evaluating lymphocyte response.Folia microbiologica · 2026Article
- Evaluation of Two Naturally Occurring Phytochemicals, 6‑Methoxy-2-benzoxazolinone and 2‑Benzoxazolinone, against Drug-ResistantACS omega · 2026Article
- Isolation and characterisation of a novel Stenotrophomonas maltophilia phage vB_SmaS_BCU-1 with evaluation of mammalian cell safety.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026Article
- Isolation and genomic characterization of a lytic bacteriophage against multidrug-resistant Proteus mirabilis.Applied microbiology and biotechnology · 2026Article
62 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Bacterial biofilms demonstrate high broad-spectrum adaptive antibiotic resistance and cause two thirds of all infections, but there is a lack of approved antibiofilm agents. Unlike the standard minimal inhibitory concentration assay to assess antibacterial activity against planktonic cells, there is no standardized method to evaluate biofilm inhibition and/or eradication capacity of novel antibiofilm compounds. The protocol described here outlines simple and reproducible methods for assessing the biofilm inhibition and eradication capacities of novel antibiofilm agents against adherent bacterial biofilms grown in 96-well microtiter plates. It employs two inexpensive dyes: crystal violet to stain adhered biofilm biomass and 2,3,5-triphenyl tetrazolium chloride to quantify metabolism of the biofilm cells. The procedure is accessible to any laboratory with a plate reader, requires minimal technical expertise or training and takes 4 or 5 d to complete. Recommendations for how biofilm inhibition and eradication results should be interpreted and presented are also described.
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33911258What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.