Evidence map›Paper›PMID 33911107›Full record

ArticleScientific reports2021

Evaluation of digital dispense-assisted broth microdilution antimicrobial susceptibility testing for Pseudomonas aeruginosa isolates.

Shawn T Clark, Patrick J Stapleton, Pauline W Wang, Yvonne C W Yau, Valerie J Waters, David M Hwang, David S Guttman

Open access · goldAbstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.3field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 2 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Shawn T ClarkDepartment of Cell & Systems Biology, University of Toronto, Toronto, ON, Canada. shawn.clark@mail.utoronto.ca.
Patrick J StapletonDepartment of Pediatric Laboratory Medicine, Division of Microbiology, The Hospital for Sick Children, Toronto, ON, Canada.
Pauline W WangCentre for the Analysis of Genome Evolution and Function, University of Toronto, Toronto, ON, Canada.
Yvonne C W YauDepartment of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada.
Valerie J WatersDepartment of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada.
David M HwangDepartment of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada.
David S GuttmanDepartment of Cell & Systems Biology, University of Toronto, Toronto, ON, Canada.
University of Toronto · CAHospital for Sick Children · CASunnybrook Health Science Centre · CA

Funding

CIHR CP-151952
6 · The paper itself

Abstract

Antimicrobial susceptibility testing (AST) is essential for detecting resistance in Pseudomonas aeruginosa and other bacterial pathogens. Here we evaluated the performance of broth microdilution (BMD) panels created using a semi-automated liquid handler, the D300e Digital Dispenser (Tecan Group Ltd., CH) that relies on inkjet printing technology. Microtitre panels (96-well) containing nine twofold dilutions of 12 antimicrobials from five classes (β-lactams, β-lactam/β-lactamase inhibitors, aminoglycosides, fluoroquinolones, polymyxins) were prepared in parallel using the D300e Digital Dispenser and standard methods described by CLSI/ISO. To assess performance, panels were challenged with three well characterized quality control organisms and 100 clinical P. aeruginosa isolates. Traditional agreement and error measures were used for evaluation. Essential (EA) and categorical (CA) agreements were 92.7% and 98.0% respectively for P. aeruginosa isolates with evaluable on-scale results. The majority of minor errors that fell outside acceptable EA parameters (≥ ± 1 dilution, 1.9%) were seen with aztreonam (5%) and ceftazidime (4%), however all antimicrobials displayed acceptable performance in this situation. Differences in MIC were often log

Indexed as

Anti-Bacterial AgentsAztreonamCefepimeCeftazidimeHumansMicrobial Sensitivity TestsPseudomonas aeruginosaPseudomonas InfectionsReproducibility of ResultsAnti-Bacterial AgentsAztreonamCefepimeCeftazidime

Identifiers

PMID33911107
PMCPMC8080699
OpenAlexW3157169657

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.