ArticleJournal of the American Society for Mass Spectrometry2021
Thiol-Cleavable Biotin for Chemical and Enzymatic Biotinylation and Its Application to Mitochondrial TurboID Proteomics.
Article in Journal of the American Society for Mass Spectrometry, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 30 citations in OpenAlex.
- Application of Proximity-Labeling Techniques in Plants: A Review of Successful Cases.International journal of molecular sciences · 2026Review
- Integrating endogenous TurboID and data-independent acquisition mass spectrometry for in vivo proximity labeling.The EMBO journal · 2026Article
- Profiling the proteome-wide selectivity of diverse electrophiles.Nature chemistry · 2025Article
- TurboID-Mediated Profiling of Glioblastoma-Derived Extracellular Vesicle Cargo Proteins.Journal of extracellular vesicles · 2025Article
- Recent advances in chemical proteomics for protein profiling and targeted degradation.Current opinion in chemical biology · 2025Review
- A Straightforward Interpretation of Proximity Labeling through Direct Biotinylation Analysis.ACS omega · 2025Article
- Overcoming Analytical Challenges in Proximity Labeling Proteomics.Journal of mass spectrometry : JMS · 2025Review
- Article
- Benchmarking and Automating the Biotinylation Proteomics Workflow.Research square · 2024Article
- Proximity Labeling in Plants.Annual review of plant biology · 2023Review
- Noninvasive evaluation of PD-L1 expression in non-small cell lung cancer by immunoPET imaging using an acylating agent-modified antibody fragment.European journal of nuclear medicine and molecular imaging · 2023Article
- A photo-oxidation driven proximity labeling strategy enables profiling of mitochondrial proteome dynamics in living cells.Chemical science · 2022Article
- Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics.Journal of visualized experiments : JoVE · 2022Article
- Molecular Spatiomics by Proximity Labeling.Accounts of chemical research · 2022Article
- Advances in enzyme-mediated proximity labeling and its potential for plant research.Plant physiology · 2022Article
- Potential application of TurboID-based proximity labeling in studying the protein interaction network in plant response to abiotic stress.Frontiers in plant science · 2022Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Protein biotinylation via chemical or enzymatic reactions is often coupled with streptavidin-based enrichment and on-bead digestion in numerous biological applications. However, the popular on-bead digestion method faces major challenges of streptavidin contamination, overwhelming signals from endogenous biotinylated proteins, the lost information on biotinylation sites, and limited sequence coverage of enriched proteins. Here, we explored thiol-cleavable biotin as an alternative approach to elute biotinylated proteins from streptavidin-coated beads for both chemical biotinylation and biotin ligase-based proximity labeling. All possible amino acid sites for biotinylation were thoroughly evaluated in addition to the primary lysine residue. We found that biotinylation at lysine residues notably reduces the trypsin digestion efficiency, which can be mitigated by the thiol-cleavable biotinylation method. We then evaluated the applicability of thiol-cleavable biotin as a substrate for proximity labeling in living cells, where TurboID biotin ligase was engineered onto the mitochondrial inner membrane facing the mitochondrial matrix. As a proof-of-principle study, thiol-cleavable biotin-assisted TurboID proteomics achieved remarkable intraorganelle spatial resolution with significantly enriched proteins localized in the mitochondrial inner membrane and mitochondrial matrix.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.