ArticleeLife2021
Electrical synaptic transmission requires a postsynaptic scaffolding protein.
Article in eLife, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 45 citations in OpenAlex.
- Defining the electrical synapse.Nature reviews. Neuroscience · 2026Review
- Uncovering the electrical synapse proteome in retinal neurons via in vivo proximity labeling.eLife · 2026Article
- Cell body clustering drives gap junction-mediated synchronous activity in command neurons.bioRxiv : the preprint server for biology · 2026Article
- Diverse roles of connexins in the neuro-vasculature in homeostasis and disease.Translational neuroscience · 2026Review
- Comparative analysis of fixation techniques for signal detection in avian embryos.Developmental biology · 2025Article
- Electrical synapse molecular diversity revealed by proximity-based proteomic discovery.bioRxiv : the preprint server for biology · 2024Article
- Regulation of Cx36 trafficking through the early secretory pathway by COPII cargo receptors and Grasp55.Cellular and molecular life sciences : CMLS · 2024Article
- Electrical Synapses Mediate Embryonic Hyperactivity in a Zebrafish Model of Fragile X Syndrome.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Article
- The components of an electrical synapse as revealed by expansion microscopy of a single synaptic contact.eLife · 2024Article
- Article
- Trafficking of Connexin36 (Cx36) in the early secretory pathway.bioRxiv : the preprint server for biology · 2024Article
- Effectiveness of fixation methods for wholemount immunohistochemistry across cellular compartments in chick embryos.bioRxiv : the preprint server for biology · 2024Article
- MicroRNA-9 promotes axon regeneration of mauthner-cell in zebrafish via her6/ calcium activity pathway.Cellular and molecular life sciences : CMLS · 2024Article
- Electrical synapse structure requires distinct isoforms of a postsynaptic scaffold.PLoS genetics · 2023Article
- Neurobeachin controls the asymmetric subcellular distribution of electrical synapse proteins.Current biology : CB · 2023Article
- Isolation of Immunocomplexes from Zebrafish Brain.Bio-protocol · 2023Article
- Neurotransmitters in Type 2 Diabetes and the Control of Systemic and Central Energy Balance.Metabolites · 2023Review
- Detecting Early Cognitive Decline in Alzheimer's Disease with Brain Synaptic Structural and Functional Evaluation.Biomedicines · 2023Review
- Channel-independent function of UNC-9/Innexin in spatial arrangement of GABAergic synapses ineLife · 2022Article
- Connexinplexity: the spatial and temporal expression of connexin genes during vertebrate organogenesis.G3 (Bethesda, Md.) · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 3 institutions in 1 country.
Funding
Abstract
Electrical synaptic transmission relies on neuronal gap junctions containing channels constructed by Connexins. While at chemical synapses neurotransmitter-gated ion channels are critically supported by scaffolding proteins, it is unknown if channels at electrical synapses require similar scaffold support. Here, we investigated the functional relationship between neuronal Connexins and Zonula Occludens 1 (ZO1), an intracellular scaffolding protein localized to electrical synapses. Using model electrical synapses in zebrafish Mauthner cells, we demonstrated that ZO1 is required for robust synaptic Connexin localization, but Connexins are dispensable for ZO1 localization. Disrupting this hierarchical ZO1/Connexin relationship abolishes electrical transmission and disrupts Mauthner cell-initiated escape responses. We found that ZO1 is asymmetrically localized exclusively postsynaptically at neuronal contacts where it functions to assemble intercellular channels. Thus, forming functional neuronal gap junctions requires a postsynaptic scaffolding protein. The critical function of a scaffolding molecule reveals an unanticipated complexity of molecular and functional organization at electrical synapses.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.