ArticleAnalytical chemistry2021
Boosting Detection of Low-Abundance Proteins in Thermal Proteome Profiling Experiments by Addition of an Isobaric Trigger Channel to TMT Multiplexes.
Article in Analytical chemistry, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Dual-pore size covalent organic frameworks shelled magnetic particles for anti-microbial peptide and microprotein enrichment with simultaneous exclusion of large proteins.Mikrochimica acta · 2026Article
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- A Resource for Exploring Regional Diversity in the Male Rat Brain Synaptic Proteome.Scientific data · 2025Article
- Quantitative Analysis of Nonhistone Lysine Methylation Sites and Lysine Demethylases in Breast Cancer Cell Lines.Journal of proteome research · 2025Article
- Immunoproteasome Activation Expands the MHC Class I Immunopeptidome, Unmasks Neoantigens, and Enhances T-cell Anti-Myeloma Activity.Molecular cancer therapeutics · 2024Article
- Single-cell multi-omics in the study of digestive system cancers.Computational and structural biotechnology journal · 2024Review
- Carrier-Guided Proteome Analysis in a High Protein Background: An Improved Approach to Host Cell Protein Identification.Journal of proteome research · 2024Article
- Quantitative analysis of non-histone lysine methylation sites and lysine demethylases in breast cancer cell lines.bioRxiv : the preprint server for biology · 2024Article
- Proteomic Profiles of Human Arterioles Isolated From Fresh Adipose Tissue or Following Overnight Storage.Laboratory investigation; a journal of technical methods and pathology · 2024Article
- Stability-based approaches in chemoproteomics.Expert reviews in molecular medicine · 2024Review
- The impact of abstinence from chronic alcohol consumption on the mouse striatal proteome: sex and subregion-specific differences.Frontiers in pharmacology · 2024Article
- Current and future directions in network biology.Bioinformatics advances · 2024Article
- Experimental strategies to improve drug-target identification in mass spectrometry-based thermal stability assays.Communications chemistry · 2023Article
- Toward the analysis of functional proteoforms using mass spectrometry-based stability proteomics.Frontiers in analytical science · 2023Article
- Boost-DiLeu: Enhanced IsobaricAnalytical chemistry · 2022Article
- Thermal Proteome Profiling Reveals the O-GlcNAc-Dependent Meltome.Journal of the American Chemical Society · 2022Article
- Scaling Up Single-Cell Proteomics.Molecular & cellular proteomics : MCP · 2022Article
- Inflect: Optimizing Computational Workflows for Thermal Proteome Profiling Data Analysis.Journal of proteome research · 2021Article
Corrections and comments
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6 authors.
Funding
Abstract
The study of low-abundance proteins is a challenge to discovery-based proteomics. Mass spectrometry (MS) applications, such as thermal proteome profiling (TPP), face specific challenges in the detection of the whole proteome as a consequence of the use of nondenaturing extraction buffers. TPP is a powerful method for the study of protein thermal stability, but quantitative accuracy is highly dependent on consistent detection. Therefore, TPP can be limited in its amenability to study low-abundance proteins that tend to have stochastic or poor detection by MS. To address this challenge, we incorporated an affinity-purified protein complex sample at submolar concentrations as an isobaric trigger channel into a mutant TPP (mTPP) workflow to provide reproducible detection and quantitation of the low-abundance subunits of the cleavage and polyadenylation factor (CPF) complex. The inclusion of an isobaric protein complex trigger channel increased detection an average of 40× for previously detected subunits and facilitated detection of CPF subunits that were previously below the limit of detection. Importantly, these gains in CPF detection did not cause large changes in melt temperature (
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