ArticlemSystems2021
Evaluation of the Effect of Storage Methods on Fecal, Saliva, and Skin Microbiome Composition.
Article in mSystems, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
51 citing papers in PubMed, 74 citations in OpenAlex.
- Modulating the human gut microbiome and health markers through kombucha consumption: a controlled clinical study.Scientific reports · 2024Trial
- Five-country analysis of geographic and dietary drivers of gut microbiome composition and genomic variation.Nature communications · 2026Article
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- Ecological and methodological insights from genetic and coprological profiling of gastrointestinal communities in wild howler monkeys.Scientific reports · 2026Article
- Bacterial Community Composition and Functional Potential of the Kleptoplastic Sea SlugBiomolecules · 2026Article
- S'Wipe: user-friendly stool collection for high-throughput gut metabolomics and multi-omics.mSystems · 2026Article
- A human-derived Bacteroides strain attenuates depressive-like behavior in a rat model of social defeat-induced stress.BMC medicine · 2026Article
- A Dangerous Prey Fish?Ecology and evolution · 2026Article
- Standardized and batch effect-independent technologies enable global collaboration in microbiome research.The ISME journal · 2026Article
- Optimising Vaginal Microbiome Profiling for Clinical Translation: A Comparative Assessment of Sample Storage Methods and a Vagina-Specific 16S rRNA Gene Database.Microorganisms · 2026Article
- Fecal Sample Collection for Gut Microbiome Research in a Prospective Cohort: A Pilot Study within the Australian Breakthrough Cancer Study.Cancer research communications · 2026Article
- Characterization of the gut microbiome of wildFrontiers in microbiomes · 2026Article
- Streamlined extraction of nucleic acids and metabolites from low- and high-biomass samples using isopropanol and matrix tubes.Microbiology spectrum · 2025Article
- Gut microbiome compositional and functional features associate with Alzheimer's disease pathology.Alzheimer's & dementia : the journal of the Alzheimer's Association · 2025Article
- Diet-Gut Microbiota Relations: Critical Appraisal of Evidence From Studies Using Metagenomics.Nutrition reviews · 2025Review
- Field expedient stool collection methods for gut microbiome analysis in deployed military environments.mSphere · 2025Article
- Distal gut colonization by oral bacteria during intensive chemotherapy: direct evidence from strain-level analysis of paired samples.NPJ biofilms and microbiomes · 2025Article
- Human Untargeted Metabolomics in High-Throughput Gut Microbiome Research: Ethanol vs Methanol.Analytical chemistry · 2025Article
- Cardiometabolic disease risk in gorillas is associated with altered gut microbial metabolism.NPJ biofilms and microbiomes · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 1 institution in 1 country.
Funding
Abstract
As the number of human microbiome studies expand, it is increasingly important to identify cost-effective, practical preservatives that allow for room temperature sample storage. Here, we reanalyzed 16S rRNA gene amplicon sequencing data from a large sample storage study published in 2016 and performed shotgun metagenomic sequencing on remnant DNA from this experiment. Both results support the initial findings that 95% ethanol, a nontoxic, cost-effective preservative, is effective at preserving samples at room temperature for weeks. We expanded on this analysis by collecting a new set of fecal, saliva, and skin samples to determine the optimal ratio of 95% ethanol to sample. We identified optimal collection protocols for fecal samples (storing a fecal swab in 95% ethanol) and saliva samples (storing unstimulated saliva in 95% ethanol at a ratio of 1:2). Storing skin swabs in 95% ethanol reduced microbial biomass and disrupted community composition, highlighting the difficulties of low biomass sample preservation. The results from this study identify practical solutions for large-scale analyses of fecal and oral microbial communities.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.