Evidence map›Paper›PMID 33906300›Full record

ArticleAsian Pacific journal of cancer prevention : APJCP2021

Anticancer Activity of Combretum fragrans F. Hoffm on Glioblastoma and Prostate Cancer Cell Lines.

Isaac Silvère Gade, Richard Tagne Simo, Corinne Chadeneau, Paule Seite, Brigitte Vannier, Alex De Theodore Atchade, Paul F Seke Etet, Emmanuel Talla, Armel H Nwabo Kamdje, Jean-Marc Muller

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Asian Pacific journal of cancer prevention : APJCP, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Isaac Silvère GadeDepartment of Organic Chemistry, Faculty of Science, University of Yaoundé I, Yaoundé, Cameroon.
Richard Tagne SimoDepartment of Biomedical Sciences, Faculty of Science, University of Ngaoundere, Ngaoundéré, Cameroon.
Corinne ChadeneauDepartment of Chemistry, Faculty of Science, University of Ngaoundere, Ngaoundéré, Cameroon.
Paule SeiteUFR Sciences Fondamentales et Appliquées, Team "Récepteurs, Régulations, Cellules Tumorales" (2RCT)-EA 3842 CAPTuR, Pôle Biologie Santé-Bât. B36/B37, University of Poitiers, 1 rue Georges Bonnet-TSA, France.
Brigitte VannierUFR Sciences Fondamentales et Appliquées, Team "Récepteurs, Régulations, Cellules Tumorales" (2RCT)-EA 3842 CAPTuR, Pôle Biologie Santé-Bât. B36/B37, University of Poitiers, 1 rue Georges Bonnet-TSA, France.
Alex De Theodore AtchadeDepartment of Organic Chemistry, Faculty of Science, University of Yaoundé I, Yaoundé, Cameroon.
Paul F Seke EtetDepartment of Physiological Sciences and Biochemistry, FMBS, University of Ngaoundere, Garoua, Cameroon.
Emmanuel TallaDepartment of Chemistry, Faculty of Science, University of Ngaoundere, Ngaoundéré, Cameroon.
Armel H Nwabo KamdjeDepartment of Biomedical Sciences, Faculty of Science, University of Ngaoundere, Ngaoundéré, Cameroon.
Jean-Marc MullerUFR Sciences Fondamentales et Appliquées, Team "Récepteurs, Régulations, Cellules Tumorales" (2RCT)-EA 3842 CAPTuR, Pôle Biologie Santé-Bât. B36/B37, University of Poitiers, 1 rue Georges Bonnet-TSA, France.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundCancer incidence has been growing in an alarming rate worldwide and new therapeutics are needed, particularly for intractable and chemoresistant cases. We evaluated the cytotoxic effects of Combretum fragrans F. Hoffm (Combretaceae) on glioblastoma (U87MG and C6) and prostate (PC-3) cancer cell lines.

methodsThe cytotoxic effect of the methanolic extract of the stem bark of Combretum fragrans was assessed using XTT (2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide) test. Expressions of Akt and ERK1/2 were determined using Western blot technique, while Caspase-3/7 kits were used to evaluate caspase-3/7 activity.

resultsC. fragrans extract inhibited the proliferation of U87 (IC50 = 20.13 µg/mL), C6 (IC50 = 12.17 µg/mL), and PC-3 (IC50 = 11.50 µg/mL) cells. Treatment with the extract resulted in lower levels (p < 0.001) of phospho-ERK1/2 and phospho-Akt in U87 cells, and instead, higher levels of phospho-ERK1/2 (p < 0.001) in C6 and PC-3 cells. An increase in caspase-3/7 activity was observed, mainly after 24 hours of treatment, indicating the activation of apoptotic processes.

conclusionAltogether, these results suggest that C. fragrans have potent anticancer properties. This plant should be further investigated for developing new anticancer drugs.

Indexed as

CombretumApoptosisBiomarkers, TumorCell Line, TumorCell ProliferationGlioblastomaHumansMalePlant ExtractsPlant StemsProstatic NeoplasmsBiomarkers, TumorPlant ExtractsAktApoptosiscancer cellsCombretum fragransERK1/2

Identifiers

PMID33906300
PMCPMC8325120

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.