Evidence map›Paper›PMID 33905521›Full record

ArticleMolecular human reproduction2021

Oocyte-secreted factors strongly stimulate sFRP4 expression in human cumulus cells.

Sahar Esfandyari, Nicola J Winston, Michelle A Fierro, Humberto Scoccia, Carlos Stocco

Open access · greenAbstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular human reproduction, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Sahar EsfandyariDepartment of Physiology and Biophysics, University of Illinois at Chicago, College of Medicine, Chicago, IL, USA.
Nicola J WinstonDivision of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, University of Illinois at Chicago, College of Medicine, Chicago, IL, USA.
Michelle A FierroDivision of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, University of Illinois at Chicago, College of Medicine, Chicago, IL, USA.
Humberto ScocciaDivision of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, University of Illinois at Chicago, College of Medicine, Chicago, IL, USA.
Carlos StoccoDepartment of Physiology and Biophysics, University of Illinois at Chicago, College of Medicine, Chicago, IL, USA.ORCID 0000-0001-7961-6681
University of Illinois Chicago · US

Funding

Salt-Inducible Kinase Regulation of Ovarian Granulosa CellsR01HD097202 · NICHD · UNIVERSITY OF ILLINOIS AT CHICAGO · PI STOCCO, CARLOS OSCAR · 2019 to 2023
$1.7M
NICHD NIH HHS R01 HD097202
6 · The paper itself

Abstract

Secreted frizzled-related protein-4 (SFRP4) belongs to a family of soluble ovarian-expressed proteins that participate in female reproduction, particularly in rodents. In humans, SFRP4 is highly expressed in cumulus cells (CCs). However, the mechanisms that stimulate SFRP4 in CCs have not been examined. We hypothesise that oocyte-secreted factors such as growth differentiation factor 9 (GDF9) and bone morphogenetic protein 15 (BMP15) are involved in the regulation of SFRP4. Human CCs were collected from patients undergoing fertility treatments and treated with GDF9 or BMP15 or their combination in the presence of FSH or vehicle. FSH treatment significantly decreased SFRP4 mRNA levels when compared with nontreated cells. However, SFRP4 mRNA levels were increased significantly by GDF9 plus BMP15 in a concentration-dependent manner in the presence or absence of FSH. The combination of GDF9 plus BMP15 also increased SFRP4 protein levels and decreased the activity of the β-catenin/T cell factor-responsive promoter significantly. GDF9 plus BMP15 inhibited steroidogenic acute regulatory protein and LH/hCG receptor stimulation by FSH, while treatment with SFRP4 blocked the stimulatory effect of FSH on these genes. The evidence demonstrates that GDF9 and BMP15 act in coordination to stimulate SFRP4 expression and suggests that SFRP4 mediates the anti-luteinising effects of the oocyte in human CCs.

Indexed as

Bone Morphogenetic Protein 15Cells, CulturedCumulus CellsDose-Response Relationship, DrugDrug SynergismFemaleFollicle Stimulating HormoneGene Expression RegulationGenes, ReporterGrowth Differentiation Factor 9HumansIntercellular Signaling Peptides and ProteinsOocytesPhosphoproteinsPrimary Cell CultureProto-Oncogene ProteinsBMP15 protein, humanBone Morphogenetic Protein 15Follicle Stimulating HormoneGrowth Differentiation Factor 9Intercellular Signaling Peptides and ProteinsLHCGR protein, humanPhosphoproteinsProto-Oncogene ProteinsReceptors, LHRNA, MessengerSecreted Frizzled-Related ProteinsSFRP4 protein, humanSteroidogenic Acute Regulatory Proteinbone morphogenetic protein 15female reproductiongrowth differentiation factor 9human cumulus cellsoocyte-secreted factorssecreted frizzled-related protein-4

Identifiers

PMID33905521
PMCPMC8164821
OpenAlexW3158097640

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.