ArticleParasitology research2021
Next-generation sequencing reveals wide genetic diversity of Blastocystis subtypes in chickens including potentially zoonotic subtypes.
Article in Parasitology research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Current global status, subtype distribution and zoonotic significance of Blastocystis in dogs and cats: a systematic review and meta-analysis.Parasites & vectors · 2022Pooled it
- First survey on the occurrence, subtype distribution and circulation of the enteric protozoan Blastocystis sp. in French broiler chicken farms.Poultry science · 2026Article
- Detection and molecular characterization of Blastocystis sp., Enterocytozoon bieneusi and Giardia duodenalis in asymptomatic animals in southeastern Iran.Scientific reports · 2025Article
- A cross-sectional survey ofOne health (Amsterdam, Netherlands) · 2024Article
- New Haplotypes of Blastocystis sp. Identified in Faeces from Various Animal Groups in Algeria.Acta parasitologica · 2024Article
- Blastocystis colonization and associations with population parameters in Thai adults.PLoS neglected tropical diseases · 2024Article
- Molecular detection and genotyping of Dientamoeba fragilis and Blastocystis sp. in housefly Musca domestica (Diptera: Muscidae): first report for Dientamoeba fragilis.Parasitology research · 2024Article
- Modern technologies and solutions to enhance surveillance and response systems for emerging zoonotic diseases.Science in One Health · 2024Review
- Systematic Review and Meta-Analysis: Epidemiology of HumanTropical medicine and infectious disease · 2023Review
- Exploring Blastocystis genetic diversity in rural schoolchildren from Colombia using next-generation amplicon sequencing reveals significant associations between contact with animals and infection risk.Parasitology research · 2023Article
- A longitudinal study of Blastocystis in dairy calves from birth through 24 months demonstrates dynamic shifts in infection rates and subtype prevalence and diversity by age.Parasites & vectors · 2023Article
- Distribution and Phylogenetic Analysis of Subtypes and Alleles of Blastocystis sp. in the Stool Samples Collected from Patients with Gastrointestinal Complaints in İzmir, Turkey.Acta parasitologica · 2023Article
- Circulation and colonisation ofEpidemiology and infection · 2023Article
- Survey of Zoonotic Diarrheagenic Protist and Hepatitis E Virus in Wild Boar (Animals : an open access journal from MDPI · 2023Article
- An update on the distribution ofHeliyon · 2022Article
- Subtypes and phylogenetic analysis of Blastocystis sp. isolates from West Ismailia, Egypt.Scientific reports · 2022Article
- Changes in Gut Microbiota Composition Associated with the Presence of Enteric ProtistMicrobiology spectrum · 2022Article
- Experimental colonization with Blastocystis ST4 is associated with protective immune responses and modulation of gut microbiome in a DSS-induced colitis mouse model.Cellular and molecular life sciences : CMLS · 2022Article
- Interactions between Blastocystis subtype ST4 and gut microbiota in vitro.Parasites & vectors · 2022Article
- Detection, Molecular Identification and Transmission of the Intestinal ProtozoaMicroorganisms · 2022Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Blastocystis sp. is an intestinal protist parasite commonly found in the feces of humans and animals worldwide. Blastocystis exhibits extensive genetic diversity and has been identified in humans and a variety of animals including other mammals and birds. Blastocystis subtypes do not exhibit strict host specificity which raises the possibility of zoonotic transmission through either direct contact or fecal contamination of food or water. However, reports detailing the subtypes and prevalence of Blastocystis in avian species are limited. Therefore, this study investigated the presence of Blastocystis in chickens by molecular characterization of the small subunit rRNA (SSU rRNA) gene. Fecal samples from 130 chickens were collected from local markets in Uberlândia and Belo Horizonte in the state of Minas Gerais, Brazil. To detect and identify subtypes of Blastocystis, a next-generation amplicon sequencing protocol was used. Forty-four of the 130 (33.8%) chickens examined were positive for Blastocystis. Blastocystis subtypes ST6 (23/130; 17.7%), ST7 (43/130; 33.1%), ST10 (1/130; 0.8%), ST14 (5/130; 3.8%), ST25 (1/130; 0.8%), and a novel subtype (ST29) (2/130; 1.5%) were observed. A nanopore sequencing strategy was used to obtain the near full-length SSU rRNA gene nucleotide sequence and validate novel subtype ST29. Mixed infections containing multiple subtypes were common and identified in 63.6% of Blastocystis-positive chickens. All positive samples contained one or both potentially zoonotic subtypes ST6 and ST7. The prevalence of Blastocystis in chickens was high, and molecular characterization mostly identified subtypes previously found in humans. Thus, chickens may be a source of human infection and environmental contamination.
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Registered trials
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