ArticleeLife2021
Single mosquito metatranscriptomics identifies vectors, emerging pathogens and reservoirs in one assay.
Article in eLife, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 84 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
84 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Fungal microbiota diversity inWellcome open research · 2026Pooled it
- Metaviromic profiling of mosquito excreta using superhydrophobic collection devices expands the known RNA virome of North America.Microbiology spectrum · 2026Article
- Virome of neotropical Sabethes mosquitoes reveals two novel viruses in the Spiciviridae family.Virus genes · 2026Article
- Small RNA genomics of Aedes aegypti mosquitoes discovers infectious viruses that trigger an RNA interference response.Nature communications · 2026Article
- Integrating metagenomics and metatranscriptomics intoThe Journal of general virology · 2026Review
- Host and geography shape microbial communities in Kenyan mosquitoes: insights from metatranscriptomics.mSystems · 2026Article
- RNA metagenomic profiling of mosquito viromes associated with Vector-Borne diseases in Quebec, Canada.PloS one · 2026Article
- Extreme GC3 codon bias in a novel brown seaweed virus results in pseudoambigrammatic characteristics.Virus evolution · 2026Article
- Viral metagenomics in mosquitoes as potential vectors of arboviruses in the Colombian Caribbean: characterisation of a "core" regional RNA virome.Memorias do Instituto Oswaldo Cruz · 2026Article
- RNA virome comparison between sylvatic and urban-interface mosquitoes from Southeastern Brazil.Frontiers in cellular and infection microbiology · 2026Article
- Putative Novel Viruses in the FamiliesAdvances in virology · 2026Article
- Mosquito viromes across England and Wales reveal hidden arbovirus signals and limited ecological structuring.Frontiers in microbiology · 2026Article
- Seasonal dynamics and species diversity of Anopheles mosquitoes in malaria endemic districts of Southern Odisha India.Scientific reports · 2025Article
- First isolation and full-length genome analysis of a new RNA virus, Wenzhou sobemo-like virus 4 fromVirulence · 2025Article
- Heritable viral symbionts in the familyApplied and environmental microbiology · 2025Article
- Virome profiling of Culex tarsalis through small RNA-seq: A challenge of suboptimal samples.PLoS neglected tropical diseases · 2025Article
- An All-in-One Metabarcoding Approach to Mosquito and Arbovirus Xenosurveillance.Molecular ecology resources · 2025Article
- Metagenomics reveals cryptic circulation of zoonotic viruses in Nigeria.Research square · 2025Article
- SegFinder: an automated tool for identifying complete RNA virus genome segments through co-occurrence in multiple sequenced samples.Briefings in bioinformatics · 2025Article
- Unveiling viral diversity and dynamics in mosquitoes through metagenomic analysis in Guizhou Province, China.Infectious diseases of poverty · 2025Article
24 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
Mosquitoes are major infectious disease-carrying vectors. Assessment of current and future risks associated with the mosquito population requires knowledge of the full repertoire of pathogens they carry, including novel viruses, as well as their blood meal sources. Unbiased metatranscriptomic sequencing of individual mosquitoes offers a straightforward, rapid, and quantitative means to acquire this information. Here, we profile 148 diverse wild-caught mosquitoes collected in California and detect sequences from eukaryotes, prokaryotes, 24 known and 46 novel viral species. Importantly, sequencing individuals greatly enhanced the value of the biological information obtained. It allowed us to (a) speciate host mosquito, (b) compute the prevalence of each microbe and recognize a high frequency of viral co-infections, (c) associate animal pathogens with specific blood meal sources, and (d) apply simple co-occurrence methods to recover previously undetected components of highly prevalent segmented viruses. In the context of emerging diseases, where knowledge about vectors, pathogens, and reservoirs is lacking, the approaches described here can provide actionable information for public health surveillance and intervention decisions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.