Evidence map›Paper›PMID 33904018›Full record

ArticleIn vitro cellular & developmental biology. Animal2021

In vitro cytotoxic effect of a chitin-like polysaccharide produced by Mortierella alpina on adrenocortical carcinoma cells H295R, and its use as mitotane adjuvant.

Luis Daniel Goyzueta Mamani, Júlio Cesar de Carvalho, Sandro J R Bonatto, Valcineide A O Tanobe, Carlos Ricardo Soccol

Abstract read
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In one paragraph

Article in In vitro cellular & developmental biology. Animal, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 2 countries.

Luis Daniel Goyzueta MamaniDepartment of Bioprocess Engineering and Biotechnology, Federal University of Paraná, P.O. box 19011, Curitiba, Paraná, 81531-990, Brazil.
Júlio Cesar de CarvalhoDepartment of Bioprocess Engineering and Biotechnology, Federal University of Paraná, P.O. box 19011, Curitiba, Paraná, 81531-990, Brazil. jccarvalho@ufpr.br.ORCID http://orcid.org/0000-0001-7558-7748
Sandro J R BonattoInstituto de Pesquisa Pelé Pequeno Príncipe, Curitiba, Brazil.
Valcineide A O TanobeDepartment of Chemistry, Centro Universitario de Ciencias Exactas e Ingenierías-CUCEI. C.P.44430, Guadalajara University, Guadalajara, Jalisco, Mexico.
Carlos Ricardo SoccolDepartment of Bioprocess Engineering and Biotechnology, Federal University of Paraná, P.O. box 19011, Curitiba, Paraná, 81531-990, Brazil.
Universidade Federal do Paraná · BRInstituto de Tecnologia do Paraná · BRUniversidad de Guadalajara · MX

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study presents an in vitro evaluation of the antitumor potential of a chitin-like exopolysaccharide (EPS, produced by Mortierella alpina) on Adrenocortical carcinoma cells (ACC) compared to mitotane, a commercial drug commonly used in ACC treatment, and known for its side effects. Techniques of cellular viability determination such as MTT and fluorescence were used to measure the cytotoxic effects of the EPS and mitotane in tumoral cells (H295R) and non-tumoral cells (VERO), observing high cytotoxicity of mitotane and a 10% superior pro-apoptotic effect of the EPS compared to mitotane (p < 0.05). The cytotoxic effect of the EPS was similar to the effect of 50 μM mitotane on tumoral cells (p < 0.05). A decrement of the lysosomal volume was also noted in tumoral cells treated with the EPS. To enhance the antitumor effect, a combination of mitotane at a lower dosage and the EPS (as adjuvant) was also tested, showing a slight improvement of the cytotoxicity effect on tumoral cells. Therefore, the results indicate a cytotoxic effect of the EPS produced by Mortierella alpina on adrenocortical carcinoma, and a possible application in biomedical formulations or additional treatments.

Indexed as

Adrenocortical CarcinomaAnimalsCell Line, TumorCell ProliferationChitinChlorocebus aethiopsHumansMitotaneMortierellaPolysaccharidesVero CellsChitinMitotanePolysaccharidesAdrenocortical carcinomaAnti-tumor effectExopolysaccharideMitotaneMortierella alpina

Identifiers

PMID33904018
OpenAlexW3159463754

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.